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Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses
The choice of an appropriate housekeeping gene for normalisation purposes has now become an essential requirement when designing QPCR experiments. This is of particular importance when using QPCR to measure viral and cellular gene transcription levels in the context of viral infections as viruses ca...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2216015/ https://www.ncbi.nlm.nih.gov/pubmed/18053162 http://dx.doi.org/10.1186/1743-422X-4-130 |
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author | Watson, Sarah Mercier, Sarah Bye, Chris Wilkinson, John Cunningham, Anthony L Harman, Andrew N |
author_facet | Watson, Sarah Mercier, Sarah Bye, Chris Wilkinson, John Cunningham, Anthony L Harman, Andrew N |
author_sort | Watson, Sarah |
collection | PubMed |
description | The choice of an appropriate housekeeping gene for normalisation purposes has now become an essential requirement when designing QPCR experiments. This is of particular importance when using QPCR to measure viral and cellular gene transcription levels in the context of viral infections as viruses can significantly interfere with host cell pathways, the components of which traditional housekeeping genes often encode. In this study we have determined the reliability of 10 housekeeping genes in context of four heavily studied viral infections; human immunodeficiency virus type 1, herpes simplex virus type 1, cytomegalovirus and varicella zoster virus infections using a variety of cell types and virus strains. This provides researchers of these viruses with a shortlist of potential housekeeping genes to use as normalisers for QPCR experiments. |
format | Text |
id | pubmed-2216015 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-22160152008-01-29 Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses Watson, Sarah Mercier, Sarah Bye, Chris Wilkinson, John Cunningham, Anthony L Harman, Andrew N Virol J Methodology The choice of an appropriate housekeeping gene for normalisation purposes has now become an essential requirement when designing QPCR experiments. This is of particular importance when using QPCR to measure viral and cellular gene transcription levels in the context of viral infections as viruses can significantly interfere with host cell pathways, the components of which traditional housekeeping genes often encode. In this study we have determined the reliability of 10 housekeeping genes in context of four heavily studied viral infections; human immunodeficiency virus type 1, herpes simplex virus type 1, cytomegalovirus and varicella zoster virus infections using a variety of cell types and virus strains. This provides researchers of these viruses with a shortlist of potential housekeeping genes to use as normalisers for QPCR experiments. BioMed Central 2007-12-03 /pmc/articles/PMC2216015/ /pubmed/18053162 http://dx.doi.org/10.1186/1743-422X-4-130 Text en Copyright © 2007 Watson et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Methodology Watson, Sarah Mercier, Sarah Bye, Chris Wilkinson, John Cunningham, Anthony L Harman, Andrew N Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses |
title | Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses |
title_full | Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses |
title_fullStr | Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses |
title_full_unstemmed | Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses |
title_short | Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses |
title_sort | determination of suitable housekeeping genes for normalisation of quantitative real time pcr analysis of cells infected with human immunodeficiency virus and herpes viruses |
topic | Methodology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2216015/ https://www.ncbi.nlm.nih.gov/pubmed/18053162 http://dx.doi.org/10.1186/1743-422X-4-130 |
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