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Desensitization by external Na of the cyclic AMP-dependent Na+/H+ antiporter in trout red blood cells

The erythrocytes of the trout, Salmo gairdneri, react to beta- adrenergic stimulation by activating a cyclic AMP-dependent and amiloride-sensitive Na+/H+ antiporter (see Borgese, F., F. Garcia- Romeu, and R. Motais, Journal of General Physiology, 1986, 87:551-566). The present study traces the kinet...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1988
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2216146/
https://www.ncbi.nlm.nih.gov/pubmed/2839593
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collection PubMed
description The erythrocytes of the trout, Salmo gairdneri, react to beta- adrenergic stimulation by activating a cyclic AMP-dependent and amiloride-sensitive Na+/H+ antiporter (see Borgese, F., F. Garcia- Romeu, and R. Motais, Journal of General Physiology, 1986, 87:551-566). The present study traces the kinetic behavior of the unidirectional Na fluxes after stimulation by isoproterenol. A very considerable increase (100-fold) of the unidirectional Na influx (JNa(in)) follows the addition of isoproterenol to the erythrocyte suspension. After 1.5 min, JNa(in) falls suddenly, and asymptotically diminishes toward the nonstimulated flux level. The unidirectional Na efflux (JNa(out)) proceeds according to similar kinetics. The decrease of JNa(in) and JNa(out)is not linked to either a change in the driving forces of the transported ions or a decrease of the cyclic AMP concentration but to a desensitization of the Na+/H+ antiporter. This desensitization is dependent on the external Na concentration and is not controlled by internal Na, cell swelling, or external Ca.
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spelling pubmed-22161462008-04-23 Desensitization by external Na of the cyclic AMP-dependent Na+/H+ antiporter in trout red blood cells J Gen Physiol Articles The erythrocytes of the trout, Salmo gairdneri, react to beta- adrenergic stimulation by activating a cyclic AMP-dependent and amiloride-sensitive Na+/H+ antiporter (see Borgese, F., F. Garcia- Romeu, and R. Motais, Journal of General Physiology, 1986, 87:551-566). The present study traces the kinetic behavior of the unidirectional Na fluxes after stimulation by isoproterenol. A very considerable increase (100-fold) of the unidirectional Na influx (JNa(in)) follows the addition of isoproterenol to the erythrocyte suspension. After 1.5 min, JNa(in) falls suddenly, and asymptotically diminishes toward the nonstimulated flux level. The unidirectional Na efflux (JNa(out)) proceeds according to similar kinetics. The decrease of JNa(in) and JNa(out)is not linked to either a change in the driving forces of the transported ions or a decrease of the cyclic AMP concentration but to a desensitization of the Na+/H+ antiporter. This desensitization is dependent on the external Na concentration and is not controlled by internal Na, cell swelling, or external Ca. The Rockefeller University Press 1988-04-01 /pmc/articles/PMC2216146/ /pubmed/2839593 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Desensitization by external Na of the cyclic AMP-dependent Na+/H+ antiporter in trout red blood cells
title Desensitization by external Na of the cyclic AMP-dependent Na+/H+ antiporter in trout red blood cells
title_full Desensitization by external Na of the cyclic AMP-dependent Na+/H+ antiporter in trout red blood cells
title_fullStr Desensitization by external Na of the cyclic AMP-dependent Na+/H+ antiporter in trout red blood cells
title_full_unstemmed Desensitization by external Na of the cyclic AMP-dependent Na+/H+ antiporter in trout red blood cells
title_short Desensitization by external Na of the cyclic AMP-dependent Na+/H+ antiporter in trout red blood cells
title_sort desensitization by external na of the cyclic amp-dependent na+/h+ antiporter in trout red blood cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2216146/
https://www.ncbi.nlm.nih.gov/pubmed/2839593