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Impaired Calcium Release in Cerebellar Purkinje Neurons Maintained in Culture

Cerebellar Purkinje neurons demonstrate a form of synaptic plasticity that, in acutely prepared brain slices, has been shown to require calcium release from the intracellular calcium stores through inositol trisphosphate (InsP(3)) receptors. Similar studies performed in cultured Purkinje cells, howe...

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Detalles Bibliográficos
Autores principales: Womack, Mary D., Walker, Jeffery W., Khodakhah, Kamran
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2217216/
https://www.ncbi.nlm.nih.gov/pubmed/10694261
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author Womack, Mary D.
Walker, Jeffery W.
Khodakhah, Kamran
author_facet Womack, Mary D.
Walker, Jeffery W.
Khodakhah, Kamran
author_sort Womack, Mary D.
collection PubMed
description Cerebellar Purkinje neurons demonstrate a form of synaptic plasticity that, in acutely prepared brain slices, has been shown to require calcium release from the intracellular calcium stores through inositol trisphosphate (InsP(3)) receptors. Similar studies performed in cultured Purkinje cells, however, find little evidence for the involvement of InsP(3) receptors. To address this discrepancy, the properties of InsP(3)- and caffeine-evoked calcium release in cultured Purkinje cells were directly examined. Photorelease of InsP(3) (up to 100 μM) from its photolabile caged analogue produced no change in calcium levels in 70% of cultured Purkinje cells. In the few cells where a calcium increase was detected, the response was very small and slow to peak. In contrast, the same concentration of InsP(3) resulted in large and rapidly rising calcium responses in all acutely dissociated Purkinje cells tested. Similar to InsP(3), caffeine also had little effect on calcium levels in cultured Purkinje cells, yet evoked large calcium transients in all acutely dissociated Purkinje cells tested. The results demonstrate that calcium release from intracellular calcium stores is severely impaired in Purkinje cells when they are maintained in culture. Our findings suggest that cultured Purkinje cells are an unfaithful experimental model for the study of the role of calcium release in the induction of cerebellar long term depression.
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spelling pubmed-22172162008-04-21 Impaired Calcium Release in Cerebellar Purkinje Neurons Maintained in Culture Womack, Mary D. Walker, Jeffery W. Khodakhah, Kamran J Gen Physiol Original Article Cerebellar Purkinje neurons demonstrate a form of synaptic plasticity that, in acutely prepared brain slices, has been shown to require calcium release from the intracellular calcium stores through inositol trisphosphate (InsP(3)) receptors. Similar studies performed in cultured Purkinje cells, however, find little evidence for the involvement of InsP(3) receptors. To address this discrepancy, the properties of InsP(3)- and caffeine-evoked calcium release in cultured Purkinje cells were directly examined. Photorelease of InsP(3) (up to 100 μM) from its photolabile caged analogue produced no change in calcium levels in 70% of cultured Purkinje cells. In the few cells where a calcium increase was detected, the response was very small and slow to peak. In contrast, the same concentration of InsP(3) resulted in large and rapidly rising calcium responses in all acutely dissociated Purkinje cells tested. Similar to InsP(3), caffeine also had little effect on calcium levels in cultured Purkinje cells, yet evoked large calcium transients in all acutely dissociated Purkinje cells tested. The results demonstrate that calcium release from intracellular calcium stores is severely impaired in Purkinje cells when they are maintained in culture. Our findings suggest that cultured Purkinje cells are an unfaithful experimental model for the study of the role of calcium release in the induction of cerebellar long term depression. The Rockefeller University Press 2000-03-01 /pmc/articles/PMC2217216/ /pubmed/10694261 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Womack, Mary D.
Walker, Jeffery W.
Khodakhah, Kamran
Impaired Calcium Release in Cerebellar Purkinje Neurons Maintained in Culture
title Impaired Calcium Release in Cerebellar Purkinje Neurons Maintained in Culture
title_full Impaired Calcium Release in Cerebellar Purkinje Neurons Maintained in Culture
title_fullStr Impaired Calcium Release in Cerebellar Purkinje Neurons Maintained in Culture
title_full_unstemmed Impaired Calcium Release in Cerebellar Purkinje Neurons Maintained in Culture
title_short Impaired Calcium Release in Cerebellar Purkinje Neurons Maintained in Culture
title_sort impaired calcium release in cerebellar purkinje neurons maintained in culture
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2217216/
https://www.ncbi.nlm.nih.gov/pubmed/10694261
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