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Reactive Oxygen Species Regulate Oxygen-Sensitive Potassium Flux in Rainbow Trout Erythrocytes

In the present study, we have investigated if reactive oxygen species are involved in the oxygen-dependent regulation of potassium-chloride cotransport activity in trout erythrocyte membrane. An increase in the oxygen level caused an increase in chloride-sensitive potassium transport (K(+)-Cl(−) cot...

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Autores principales: Bogdanova, Anna Yu, Nikinmaa, Mikko
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2217244/
https://www.ncbi.nlm.nih.gov/pubmed/11158169
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author Bogdanova, Anna Yu
Nikinmaa, Mikko
author_facet Bogdanova, Anna Yu
Nikinmaa, Mikko
author_sort Bogdanova, Anna Yu
collection PubMed
description In the present study, we have investigated if reactive oxygen species are involved in the oxygen-dependent regulation of potassium-chloride cotransport activity in trout erythrocyte membrane. An increase in the oxygen level caused an increase in chloride-sensitive potassium transport (K(+)-Cl(−) cotransport). 5 mM hydrogen peroxide caused an increase in K(+)-Cl(−) cotransport at 5% oxygen. The increase in flux could be inhibited by adding extracellular catalase in the incubation. Pretreatment of the cells with mercaptopropionyl glycine (MPG), a scavenger of reactive oxygen species showing preference for hydroxyl radicals, abolished the activation of the K(+)-Cl(−) cotransporter by increased oxygen levels. The inhibition by MPG was reversible, and MPG could not inhibit the activation of transporter by the sulfhydryl reagent, N-ethylmaleimide, indicating that the effect of MPG was due to the scavenging of reactive oxygen species and not to the reaction of MPG with the cotransporter. Copper ions, which catalyze the production of hydroxyl radicals in the Fenton reaction, activated K(+)-Cl(−) cotransport significantly at hypoxic conditions (1% O(2)). These data suggest that hydroxyl radicals, formed from O(2) in close vicinity to the cell membrane, play an important role in the oxygen-dependent activation of the K(+)-Cl(−) cotransporter.
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spelling pubmed-22172442008-04-22 Reactive Oxygen Species Regulate Oxygen-Sensitive Potassium Flux in Rainbow Trout Erythrocytes Bogdanova, Anna Yu Nikinmaa, Mikko J Gen Physiol Original Article In the present study, we have investigated if reactive oxygen species are involved in the oxygen-dependent regulation of potassium-chloride cotransport activity in trout erythrocyte membrane. An increase in the oxygen level caused an increase in chloride-sensitive potassium transport (K(+)-Cl(−) cotransport). 5 mM hydrogen peroxide caused an increase in K(+)-Cl(−) cotransport at 5% oxygen. The increase in flux could be inhibited by adding extracellular catalase in the incubation. Pretreatment of the cells with mercaptopropionyl glycine (MPG), a scavenger of reactive oxygen species showing preference for hydroxyl radicals, abolished the activation of the K(+)-Cl(−) cotransporter by increased oxygen levels. The inhibition by MPG was reversible, and MPG could not inhibit the activation of transporter by the sulfhydryl reagent, N-ethylmaleimide, indicating that the effect of MPG was due to the scavenging of reactive oxygen species and not to the reaction of MPG with the cotransporter. Copper ions, which catalyze the production of hydroxyl radicals in the Fenton reaction, activated K(+)-Cl(−) cotransport significantly at hypoxic conditions (1% O(2)). These data suggest that hydroxyl radicals, formed from O(2) in close vicinity to the cell membrane, play an important role in the oxygen-dependent activation of the K(+)-Cl(−) cotransporter. The Rockefeller University Press 2001-02-01 /pmc/articles/PMC2217244/ /pubmed/11158169 Text en © 2001 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Bogdanova, Anna Yu
Nikinmaa, Mikko
Reactive Oxygen Species Regulate Oxygen-Sensitive Potassium Flux in Rainbow Trout Erythrocytes
title Reactive Oxygen Species Regulate Oxygen-Sensitive Potassium Flux in Rainbow Trout Erythrocytes
title_full Reactive Oxygen Species Regulate Oxygen-Sensitive Potassium Flux in Rainbow Trout Erythrocytes
title_fullStr Reactive Oxygen Species Regulate Oxygen-Sensitive Potassium Flux in Rainbow Trout Erythrocytes
title_full_unstemmed Reactive Oxygen Species Regulate Oxygen-Sensitive Potassium Flux in Rainbow Trout Erythrocytes
title_short Reactive Oxygen Species Regulate Oxygen-Sensitive Potassium Flux in Rainbow Trout Erythrocytes
title_sort reactive oxygen species regulate oxygen-sensitive potassium flux in rainbow trout erythrocytes
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2217244/
https://www.ncbi.nlm.nih.gov/pubmed/11158169
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AT nikinmaamikko reactiveoxygenspeciesregulateoxygensensitivepotassiumfluxinrainbowtrouterythrocytes