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Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes

Dihydropyridines (DHPs) are well known for their effects on L-type voltage-dependent Ca(2+) channels. However, these drugs also affect other voltage-dependent ion channels, including Shaker K(+) channels. We examined the effects of DHPs on the Shaker K(+) channels expressed in Xenopus oocytes. Intra...

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Detalles Bibliográficos
Autores principales: Avdonin, Vladimir, Shibata, Erwin F., Hoshi, Toshinori
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2220064/
https://www.ncbi.nlm.nih.gov/pubmed/9041446
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author Avdonin, Vladimir
Shibata, Erwin F.
Hoshi, Toshinori
author_facet Avdonin, Vladimir
Shibata, Erwin F.
Hoshi, Toshinori
author_sort Avdonin, Vladimir
collection PubMed
description Dihydropyridines (DHPs) are well known for their effects on L-type voltage-dependent Ca(2+) channels. However, these drugs also affect other voltage-dependent ion channels, including Shaker K(+) channels. We examined the effects of DHPs on the Shaker K(+) channels expressed in Xenopus oocytes. Intracellular applications of DHPs quickly and reversibly induced apparent inactivation in the Shaker K(+) mutant channels with disrupted N- and C-type inactivation. We found that DHPs interact with the open state of the channel as evidenced by the decreased mean open time. The DHPs effects are voltage-dependent, becoming more effective with hyperpolarization. A model which involves binding of two DHP molecules to the channel is consistent with the results obtained in our experiments.
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spelling pubmed-22200642008-04-22 Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes Avdonin, Vladimir Shibata, Erwin F. Hoshi, Toshinori J Gen Physiol Article Dihydropyridines (DHPs) are well known for their effects on L-type voltage-dependent Ca(2+) channels. However, these drugs also affect other voltage-dependent ion channels, including Shaker K(+) channels. We examined the effects of DHPs on the Shaker K(+) channels expressed in Xenopus oocytes. Intracellular applications of DHPs quickly and reversibly induced apparent inactivation in the Shaker K(+) mutant channels with disrupted N- and C-type inactivation. We found that DHPs interact with the open state of the channel as evidenced by the decreased mean open time. The DHPs effects are voltage-dependent, becoming more effective with hyperpolarization. A model which involves binding of two DHP molecules to the channel is consistent with the results obtained in our experiments. The Rockefeller University Press 1997-02-01 /pmc/articles/PMC2220064/ /pubmed/9041446 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Avdonin, Vladimir
Shibata, Erwin F.
Hoshi, Toshinori
Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes
title Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes
title_full Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes
title_fullStr Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes
title_full_unstemmed Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes
title_short Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes
title_sort dihydropyridine action on voltage-dependent potassium channels expressed in xenopus oocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2220064/
https://www.ncbi.nlm.nih.gov/pubmed/9041446
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AT hoshitoshinori dihydropyridineactiononvoltagedependentpotassiumchannelsexpressedinxenopusoocytes