Purification, crystallization and preliminary X-ray diffraction studies of N-acetylglucosamine-phosphate mutase from Candida albicans

N-acetylglucosamine-phosphate mutase (AGM1) is an essential enzyme in the synthesis of UDP-N-acetylglucosamine (UDP-GlcNAc) in eukaryotes and belongs to the α-d-phosphohexomutase superfamily. AGM1 from Candida albicans (CaAGM1) was purified and crystallized by the sitting-drop vapour-diffusion metho...

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Autores principales: Nishitani, Yuichi, Maruyama, Daisuke, Nonaka, Tsuyoshi, Kita, Akiko, Fukami, Takaaki A., Mio, Toshiyuki, Yamada-Okabe, Hisafumi, Yamada-Okabe, Toshiko, Miki, Kunio
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2006
Materias:
Crystallization Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2222579/
https://www.ncbi.nlm.nih.gov/pubmed/16582501
http://dx.doi.org/10.1107/S1744309106010177
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author Nishitani, Yuichi
Maruyama, Daisuke
Nonaka, Tsuyoshi
Kita, Akiko
Fukami, Takaaki A.
Mio, Toshiyuki
Yamada-Okabe, Hisafumi
Yamada-Okabe, Toshiko
Miki, Kunio
author_facet Nishitani, Yuichi
Maruyama, Daisuke
Nonaka, Tsuyoshi
Kita, Akiko
Fukami, Takaaki A.
Mio, Toshiyuki
Yamada-Okabe, Hisafumi
Yamada-Okabe, Toshiko
Miki, Kunio
author_sort Nishitani, Yuichi
collection PubMed
description N-acetylglucosamine-phosphate mutase (AGM1) is an essential enzyme in the synthesis of UDP-N-acetylglucosamine (UDP-GlcNAc) in eukaryotes and belongs to the α-d-phosphohexomutase superfamily. AGM1 from Candida albicans (CaAGM1) was purified and crystallized by the sitting-drop vapour-diffusion method. The crystals obtained belong to the primitive monoclinic space group P2(1), with unit-cell parameters a = 60.2, b = 130.2, c = 78.0 Å, β = 106.7°. The crystals diffract X-rays to beyond 1.8 Å resolution using synchrotron radiation.
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spelling pubmed-22225792008-03-13 Purification, crystallization and preliminary X-ray diffraction studies of N-acetylglucosamine-phosphate mutase from Candida albicans Nishitani, Yuichi Maruyama, Daisuke Nonaka, Tsuyoshi Kita, Akiko Fukami, Takaaki A. Mio, Toshiyuki Yamada-Okabe, Hisafumi Yamada-Okabe, Toshiko Miki, Kunio Acta Crystallogr Sect F Struct Biol Cryst Commun Crystallization Communications N-acetylglucosamine-phosphate mutase (AGM1) is an essential enzyme in the synthesis of UDP-N-acetylglucosamine (UDP-GlcNAc) in eukaryotes and belongs to the α-d-phosphohexomutase superfamily. AGM1 from Candida albicans (CaAGM1) was purified and crystallized by the sitting-drop vapour-diffusion method. The crystals obtained belong to the primitive monoclinic space group P2(1), with unit-cell parameters a = 60.2, b = 130.2, c = 78.0 Å, β = 106.7°. The crystals diffract X-rays to beyond 1.8 Å resolution using synchrotron radiation. International Union of Crystallography 2006-03-25 /pmc/articles/PMC2222579/ /pubmed/16582501 http://dx.doi.org/10.1107/S1744309106010177 Text en © International Union of Crystallography 2006 http://journals.iucr.org/services/termsofuse.html This is an open-access article distributed under the terms described at http://journals.iucr.org/services/termsofuse.html.
spellingShingle Crystallization Communications
Nishitani, Yuichi
Maruyama, Daisuke
Nonaka, Tsuyoshi
Kita, Akiko
Fukami, Takaaki A.
Mio, Toshiyuki
Yamada-Okabe, Hisafumi
Yamada-Okabe, Toshiko
Miki, Kunio
Purification, crystallization and preliminary X-ray diffraction studies of N-acetylglucosamine-phosphate mutase from Candida albicans
title Purification, crystallization and preliminary X-ray diffraction studies of N-acetylglucosamine-phosphate mutase from Candida albicans
title_full Purification, crystallization and preliminary X-ray diffraction studies of N-acetylglucosamine-phosphate mutase from Candida albicans
title_fullStr Purification, crystallization and preliminary X-ray diffraction studies of N-acetylglucosamine-phosphate mutase from Candida albicans
title_full_unstemmed Purification, crystallization and preliminary X-ray diffraction studies of N-acetylglucosamine-phosphate mutase from Candida albicans
title_short Purification, crystallization and preliminary X-ray diffraction studies of N-acetylglucosamine-phosphate mutase from Candida albicans
title_sort purification, crystallization and preliminary x-ray diffraction studies of n-acetylglucosamine-phosphate mutase from candida albicans
topic Crystallization Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2222579/
https://www.ncbi.nlm.nih.gov/pubmed/16582501
http://dx.doi.org/10.1107/S1744309106010177
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