Flow cytometric S-phase fraction in soft-tissue sarcoma: prognostic importance analysed in 160 patients.

We could determine the S-phase fraction (SPF) by flow cytometric DNA analysis of paraffin archival material in 160 of 260 patients with soft-tissue sarcoma of extremity and trunk wall. The prognostic value of SPF was compared with other clinicopathological factors. The median follow-up time was 16 (...

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Autores principales: Gustafson, P., Fernö, M., Akerman, M., Baldetorp, B., Willén, H., Killander, D., Rydholm, A.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1997
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2222687/
https://www.ncbi.nlm.nih.gov/pubmed/9000604
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author Gustafson, P.
Fernö, M.
Akerman, M.
Baldetorp, B.
Willén, H.
Killander, D.
Rydholm, A.
author_facet Gustafson, P.
Fernö, M.
Akerman, M.
Baldetorp, B.
Willén, H.
Killander, D.
Rydholm, A.
author_sort Gustafson, P.
collection PubMed
description We could determine the S-phase fraction (SPF) by flow cytometric DNA analysis of paraffin archival material in 160 of 260 patients with soft-tissue sarcoma of extremity and trunk wall. The prognostic value of SPF was compared with other clinicopathological factors. The median follow-up time was 16 (6-31) years. In a univariate analysis, deep tumour location, increasing tumour size and histological malignancy grade, microscopic tumour necrosis, vascular invasion, DNA non-diploidy and high SPF (>3.0%) were associated with poor metastasis-free survival. In a multivariate analysis, microscopic tumour necrosis and high SPF were independently prognostic for metastasis. Used in combination with tumour size, microscopic tumour necrosis and vascular invasion, SPF could identify a group of patients with a 5-year metastasis-free survival rate of 0.97. This group constituted one-quarter of all patients. Patients with low SPF who did recur had a prolonged clinical course both as regards metastases and local recurrence. We conclude that SPF is a valuable adjunct in prognostication in soft-tissue sarcoma.
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spelling pubmed-22226872009-09-10 Flow cytometric S-phase fraction in soft-tissue sarcoma: prognostic importance analysed in 160 patients. Gustafson, P. Fernö, M. Akerman, M. Baldetorp, B. Willén, H. Killander, D. Rydholm, A. Br J Cancer Research Article We could determine the S-phase fraction (SPF) by flow cytometric DNA analysis of paraffin archival material in 160 of 260 patients with soft-tissue sarcoma of extremity and trunk wall. The prognostic value of SPF was compared with other clinicopathological factors. The median follow-up time was 16 (6-31) years. In a univariate analysis, deep tumour location, increasing tumour size and histological malignancy grade, microscopic tumour necrosis, vascular invasion, DNA non-diploidy and high SPF (>3.0%) were associated with poor metastasis-free survival. In a multivariate analysis, microscopic tumour necrosis and high SPF were independently prognostic for metastasis. Used in combination with tumour size, microscopic tumour necrosis and vascular invasion, SPF could identify a group of patients with a 5-year metastasis-free survival rate of 0.97. This group constituted one-quarter of all patients. Patients with low SPF who did recur had a prolonged clinical course both as regards metastases and local recurrence. We conclude that SPF is a valuable adjunct in prognostication in soft-tissue sarcoma. Nature Publishing Group 1997 /pmc/articles/PMC2222687/ /pubmed/9000604 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Gustafson, P.
Fernö, M.
Akerman, M.
Baldetorp, B.
Willén, H.
Killander, D.
Rydholm, A.
Flow cytometric S-phase fraction in soft-tissue sarcoma: prognostic importance analysed in 160 patients.
title Flow cytometric S-phase fraction in soft-tissue sarcoma: prognostic importance analysed in 160 patients.
title_full Flow cytometric S-phase fraction in soft-tissue sarcoma: prognostic importance analysed in 160 patients.
title_fullStr Flow cytometric S-phase fraction in soft-tissue sarcoma: prognostic importance analysed in 160 patients.
title_full_unstemmed Flow cytometric S-phase fraction in soft-tissue sarcoma: prognostic importance analysed in 160 patients.
title_short Flow cytometric S-phase fraction in soft-tissue sarcoma: prognostic importance analysed in 160 patients.
title_sort flow cytometric s-phase fraction in soft-tissue sarcoma: prognostic importance analysed in 160 patients.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2222687/
https://www.ncbi.nlm.nih.gov/pubmed/9000604
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