Interleukin 2 exerts autocrine stimulation on murine T-cell leukaemia growth.

As it has been suggested that an autocrine mechanism may control tumour cell growth, in this work cells from a spontaneous murine T lymphocyte leukaemia (LB) expressing the interleukin-2 receptor (IL-2R) (CD25) were evaluated in vitro for IL-2-mediated autocrine growth. Cells grew readily in culture...

Descripción completa

Detalles Bibliográficos
Autores principales: Waldner, C. I., Mongini, C., Alvarez, E., Sánchez Lockhart, M., Gravisaco, M. J., Hajos, S. E.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1997
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2222760/
https://www.ncbi.nlm.nih.gov/pubmed/9083328
_version_ 1782149382987055104
author Waldner, C. I.
Mongini, C.
Alvarez, E.
Sánchez Lockhart, M.
Gravisaco, M. J.
Hajos, S. E.
author_facet Waldner, C. I.
Mongini, C.
Alvarez, E.
Sánchez Lockhart, M.
Gravisaco, M. J.
Hajos, S. E.
author_sort Waldner, C. I.
collection PubMed
description As it has been suggested that an autocrine mechanism may control tumour cell growth, in this work cells from a spontaneous murine T lymphocyte leukaemia (LB) expressing the interleukin-2 receptor (IL-2R) (CD25) were evaluated in vitro for IL-2-mediated autocrine growth. Cells grew readily in culture and proliferation was enhanced by the addition of recombinant IL-2 but inhibited by monoclonal antibodies against either IL-2 or IL-2 receptor, in the absence of exogenous IL-2. Cyclosporin A also inhibited LB cell growth. However, when exogenous IL-2 was added together with cyclosporin A, cell proliferation proved similar to controls. Using reverse transcription polymerase chain reaction (PCR), mRNA for IL-2 was found to be present in tumour cells. Our findings support the hypothesis that LB tumour cell proliferation is mediated by an autocrine pathway involving endogenous IL-2 generation, despite the fact that these cells are not dependent on exogenous IL-2 to grow in culture. IMAGES:
format Text
id pubmed-2222760
institution National Center for Biotechnology Information
language English
publishDate 1997
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-22227602009-09-10 Interleukin 2 exerts autocrine stimulation on murine T-cell leukaemia growth. Waldner, C. I. Mongini, C. Alvarez, E. Sánchez Lockhart, M. Gravisaco, M. J. Hajos, S. E. Br J Cancer Research Article As it has been suggested that an autocrine mechanism may control tumour cell growth, in this work cells from a spontaneous murine T lymphocyte leukaemia (LB) expressing the interleukin-2 receptor (IL-2R) (CD25) were evaluated in vitro for IL-2-mediated autocrine growth. Cells grew readily in culture and proliferation was enhanced by the addition of recombinant IL-2 but inhibited by monoclonal antibodies against either IL-2 or IL-2 receptor, in the absence of exogenous IL-2. Cyclosporin A also inhibited LB cell growth. However, when exogenous IL-2 was added together with cyclosporin A, cell proliferation proved similar to controls. Using reverse transcription polymerase chain reaction (PCR), mRNA for IL-2 was found to be present in tumour cells. Our findings support the hypothesis that LB tumour cell proliferation is mediated by an autocrine pathway involving endogenous IL-2 generation, despite the fact that these cells are not dependent on exogenous IL-2 to grow in culture. IMAGES: Nature Publishing Group 1997 /pmc/articles/PMC2222760/ /pubmed/9083328 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Waldner, C. I.
Mongini, C.
Alvarez, E.
Sánchez Lockhart, M.
Gravisaco, M. J.
Hajos, S. E.
Interleukin 2 exerts autocrine stimulation on murine T-cell leukaemia growth.
title Interleukin 2 exerts autocrine stimulation on murine T-cell leukaemia growth.
title_full Interleukin 2 exerts autocrine stimulation on murine T-cell leukaemia growth.
title_fullStr Interleukin 2 exerts autocrine stimulation on murine T-cell leukaemia growth.
title_full_unstemmed Interleukin 2 exerts autocrine stimulation on murine T-cell leukaemia growth.
title_short Interleukin 2 exerts autocrine stimulation on murine T-cell leukaemia growth.
title_sort interleukin 2 exerts autocrine stimulation on murine t-cell leukaemia growth.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2222760/
https://www.ncbi.nlm.nih.gov/pubmed/9083328
work_keys_str_mv AT waldnerci interleukin2exertsautocrinestimulationonmurinetcellleukaemiagrowth
AT monginic interleukin2exertsautocrinestimulationonmurinetcellleukaemiagrowth
AT alvareze interleukin2exertsautocrinestimulationonmurinetcellleukaemiagrowth
AT sanchezlockhartm interleukin2exertsautocrinestimulationonmurinetcellleukaemiagrowth
AT gravisacomj interleukin2exertsautocrinestimulationonmurinetcellleukaemiagrowth
AT hajosse interleukin2exertsautocrinestimulationonmurinetcellleukaemiagrowth

Ejemplares similares