Cargando…

A CHEMICAL AND HISTOCHEMICAL STUDY OF THE LIPIDES OF THE PYLORIC CECUM OF THE STARFISH, ASTERIAS FORBESI

The lipides of the diverticula of Asterias forbesi have been studied by histochemical and biochemical means. Correlations between results obtained by histochemical examination of sections, and chemical analysis of isolated lipide have been made, particularly with respect to phosphatides, steroids, a...

Descripción completa

Detalles Bibliográficos
Autores principales: Karnovsky, Manfred L., Jeffrey, Susan Skilling, Thompson, Michaela Smits, Deane, Helen Wendler
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1955
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223768/
https://www.ncbi.nlm.nih.gov/pubmed/14381440
_version_ 1782149435268005888
author Karnovsky, Manfred L.
Jeffrey, Susan Skilling
Thompson, Michaela Smits
Deane, Helen Wendler
author_facet Karnovsky, Manfred L.
Jeffrey, Susan Skilling
Thompson, Michaela Smits
Deane, Helen Wendler
author_sort Karnovsky, Manfred L.
collection PubMed
description The lipides of the diverticula of Asterias forbesi have been studied by histochemical and biochemical means. Correlations between results obtained by histochemical examination of sections, and chemical analysis of isolated lipide have been made, particularly with respect to phosphatides, steroids, and aldehyde lipides. The results of the histochemical study were in good agreement with the chemical data as to the nature of the phosphatide fraction, the presence of acetone-soluble aldehyde lipides, and the composition of the free droplet fat. Homogenized diverticula were differentially centrifuged in order to establish the distribution of types of lipides in the various cellular components. In addition, data have been presented which demonstrate a direct correlation between the titer of α-glycerol ethers and that of acetone-soluble lipide acetals in the unsaponifiable fraction.
format Text
id pubmed-2223768
institution National Center for Biotechnology Information
language English
publishDate 1955
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-22237682008-05-01 A CHEMICAL AND HISTOCHEMICAL STUDY OF THE LIPIDES OF THE PYLORIC CECUM OF THE STARFISH, ASTERIAS FORBESI Karnovsky, Manfred L. Jeffrey, Susan Skilling Thompson, Michaela Smits Deane, Helen Wendler J Biophys Biochem Cytol Article The lipides of the diverticula of Asterias forbesi have been studied by histochemical and biochemical means. Correlations between results obtained by histochemical examination of sections, and chemical analysis of isolated lipide have been made, particularly with respect to phosphatides, steroids, and aldehyde lipides. The results of the histochemical study were in good agreement with the chemical data as to the nature of the phosphatide fraction, the presence of acetone-soluble aldehyde lipides, and the composition of the free droplet fat. Homogenized diverticula were differentially centrifuged in order to establish the distribution of types of lipides in the various cellular components. In addition, data have been presented which demonstrate a direct correlation between the titer of α-glycerol ethers and that of acetone-soluble lipide acetals in the unsaponifiable fraction. The Rockefeller University Press 1955-03-25 /pmc/articles/PMC2223768/ /pubmed/14381440 Text en Copyright © Copyright, 1955, by The Rockefeller Institute for Medical Research
spellingShingle Article
Karnovsky, Manfred L.
Jeffrey, Susan Skilling
Thompson, Michaela Smits
Deane, Helen Wendler
A CHEMICAL AND HISTOCHEMICAL STUDY OF THE LIPIDES OF THE PYLORIC CECUM OF THE STARFISH, ASTERIAS FORBESI
title A CHEMICAL AND HISTOCHEMICAL STUDY OF THE LIPIDES OF THE PYLORIC CECUM OF THE STARFISH, ASTERIAS FORBESI
title_full A CHEMICAL AND HISTOCHEMICAL STUDY OF THE LIPIDES OF THE PYLORIC CECUM OF THE STARFISH, ASTERIAS FORBESI
title_fullStr A CHEMICAL AND HISTOCHEMICAL STUDY OF THE LIPIDES OF THE PYLORIC CECUM OF THE STARFISH, ASTERIAS FORBESI
title_full_unstemmed A CHEMICAL AND HISTOCHEMICAL STUDY OF THE LIPIDES OF THE PYLORIC CECUM OF THE STARFISH, ASTERIAS FORBESI
title_short A CHEMICAL AND HISTOCHEMICAL STUDY OF THE LIPIDES OF THE PYLORIC CECUM OF THE STARFISH, ASTERIAS FORBESI
title_sort chemical and histochemical study of the lipides of the pyloric cecum of the starfish, asterias forbesi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223768/
https://www.ncbi.nlm.nih.gov/pubmed/14381440
work_keys_str_mv AT karnovskymanfredl achemicalandhistochemicalstudyofthelipidesofthepyloriccecumofthestarfishasteriasforbesi
AT jeffreysusanskilling achemicalandhistochemicalstudyofthelipidesofthepyloriccecumofthestarfishasteriasforbesi
AT thompsonmichaelasmits achemicalandhistochemicalstudyofthelipidesofthepyloriccecumofthestarfishasteriasforbesi
AT deanehelenwendler achemicalandhistochemicalstudyofthelipidesofthepyloriccecumofthestarfishasteriasforbesi
AT karnovskymanfredl chemicalandhistochemicalstudyofthelipidesofthepyloriccecumofthestarfishasteriasforbesi
AT jeffreysusanskilling chemicalandhistochemicalstudyofthelipidesofthepyloriccecumofthestarfishasteriasforbesi
AT thompsonmichaelasmits chemicalandhistochemicalstudyofthelipidesofthepyloriccecumofthestarfishasteriasforbesi
AT deanehelenwendler chemicalandhistochemicalstudyofthelipidesofthepyloriccecumofthestarfishasteriasforbesi