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A CHEMICAL AND HISTOCHEMICAL STUDY OF THE LIPIDES OF THE PYLORIC CECUM OF THE STARFISH, ASTERIAS FORBESI
The lipides of the diverticula of Asterias forbesi have been studied by histochemical and biochemical means. Correlations between results obtained by histochemical examination of sections, and chemical analysis of isolated lipide have been made, particularly with respect to phosphatides, steroids, a...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1955
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223768/ https://www.ncbi.nlm.nih.gov/pubmed/14381440 |
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author | Karnovsky, Manfred L. Jeffrey, Susan Skilling Thompson, Michaela Smits Deane, Helen Wendler |
author_facet | Karnovsky, Manfred L. Jeffrey, Susan Skilling Thompson, Michaela Smits Deane, Helen Wendler |
author_sort | Karnovsky, Manfred L. |
collection | PubMed |
description | The lipides of the diverticula of Asterias forbesi have been studied by histochemical and biochemical means. Correlations between results obtained by histochemical examination of sections, and chemical analysis of isolated lipide have been made, particularly with respect to phosphatides, steroids, and aldehyde lipides. The results of the histochemical study were in good agreement with the chemical data as to the nature of the phosphatide fraction, the presence of acetone-soluble aldehyde lipides, and the composition of the free droplet fat. Homogenized diverticula were differentially centrifuged in order to establish the distribution of types of lipides in the various cellular components. In addition, data have been presented which demonstrate a direct correlation between the titer of α-glycerol ethers and that of acetone-soluble lipide acetals in the unsaponifiable fraction. |
format | Text |
id | pubmed-2223768 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1955 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22237682008-05-01 A CHEMICAL AND HISTOCHEMICAL STUDY OF THE LIPIDES OF THE PYLORIC CECUM OF THE STARFISH, ASTERIAS FORBESI Karnovsky, Manfred L. Jeffrey, Susan Skilling Thompson, Michaela Smits Deane, Helen Wendler J Biophys Biochem Cytol Article The lipides of the diverticula of Asterias forbesi have been studied by histochemical and biochemical means. Correlations between results obtained by histochemical examination of sections, and chemical analysis of isolated lipide have been made, particularly with respect to phosphatides, steroids, and aldehyde lipides. The results of the histochemical study were in good agreement with the chemical data as to the nature of the phosphatide fraction, the presence of acetone-soluble aldehyde lipides, and the composition of the free droplet fat. Homogenized diverticula were differentially centrifuged in order to establish the distribution of types of lipides in the various cellular components. In addition, data have been presented which demonstrate a direct correlation between the titer of α-glycerol ethers and that of acetone-soluble lipide acetals in the unsaponifiable fraction. The Rockefeller University Press 1955-03-25 /pmc/articles/PMC2223768/ /pubmed/14381440 Text en Copyright © Copyright, 1955, by The Rockefeller Institute for Medical Research |
spellingShingle | Article Karnovsky, Manfred L. Jeffrey, Susan Skilling Thompson, Michaela Smits Deane, Helen Wendler A CHEMICAL AND HISTOCHEMICAL STUDY OF THE LIPIDES OF THE PYLORIC CECUM OF THE STARFISH, ASTERIAS FORBESI |
title | A CHEMICAL AND HISTOCHEMICAL STUDY OF THE LIPIDES OF THE PYLORIC CECUM OF THE STARFISH, ASTERIAS FORBESI |
title_full | A CHEMICAL AND HISTOCHEMICAL STUDY OF THE LIPIDES OF THE PYLORIC CECUM OF THE STARFISH, ASTERIAS FORBESI |
title_fullStr | A CHEMICAL AND HISTOCHEMICAL STUDY OF THE LIPIDES OF THE PYLORIC CECUM OF THE STARFISH, ASTERIAS FORBESI |
title_full_unstemmed | A CHEMICAL AND HISTOCHEMICAL STUDY OF THE LIPIDES OF THE PYLORIC CECUM OF THE STARFISH, ASTERIAS FORBESI |
title_short | A CHEMICAL AND HISTOCHEMICAL STUDY OF THE LIPIDES OF THE PYLORIC CECUM OF THE STARFISH, ASTERIAS FORBESI |
title_sort | chemical and histochemical study of the lipides of the pyloric cecum of the starfish, asterias forbesi |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223768/ https://www.ncbi.nlm.nih.gov/pubmed/14381440 |
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