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THYMINE IN THE ACID-SOLUBLE FRACTION OF ARBACIA EGGS
Mature Arbacia eggs were extracted with cold dilute perchloric acid, the extract concentrated, and the concentrate digested in hot perchloric acid. Thymine was recovered from the digest by paper chromatography, and the amount per egg found to be about 5 times the amount per sperm. This was the amoun...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1955
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223772/ https://www.ncbi.nlm.nih.gov/pubmed/14381439 |
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author | Marshak, A. Marshak, C. |
author_facet | Marshak, A. Marshak, C. |
author_sort | Marshak, A. |
collection | PubMed |
description | Mature Arbacia eggs were extracted with cold dilute perchloric acid, the extract concentrated, and the concentrate digested in hot perchloric acid. Thymine was recovered from the digest by paper chromatography, and the amount per egg found to be about 5 times the amount per sperm. This was the amount expected from previous experiments and is believed to represent all or almost all of the thymine in the egg. The result supports previous observations that DNA is absent from the mature egg although present in the nucleus of the egg in the germinal vesicle stage. No thymine could be recovered from a similar extract of 5,000 times as many sperm of the same species. The observations are consistent with the theory that DNA and its derivatives act as metabolic antagonists of the corresponding ribose compounds. |
format | Text |
id | pubmed-2223772 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1955 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22237722008-05-01 THYMINE IN THE ACID-SOLUBLE FRACTION OF ARBACIA EGGS Marshak, A. Marshak, C. J Biophys Biochem Cytol Article Mature Arbacia eggs were extracted with cold dilute perchloric acid, the extract concentrated, and the concentrate digested in hot perchloric acid. Thymine was recovered from the digest by paper chromatography, and the amount per egg found to be about 5 times the amount per sperm. This was the amount expected from previous experiments and is believed to represent all or almost all of the thymine in the egg. The result supports previous observations that DNA is absent from the mature egg although present in the nucleus of the egg in the germinal vesicle stage. No thymine could be recovered from a similar extract of 5,000 times as many sperm of the same species. The observations are consistent with the theory that DNA and its derivatives act as metabolic antagonists of the corresponding ribose compounds. The Rockefeller University Press 1955-03-25 /pmc/articles/PMC2223772/ /pubmed/14381439 Text en Copyright © Copyright, 1955, by The Rockefeller Institute for Medical Research |
spellingShingle | Article Marshak, A. Marshak, C. THYMINE IN THE ACID-SOLUBLE FRACTION OF ARBACIA EGGS |
title | THYMINE IN THE ACID-SOLUBLE FRACTION OF ARBACIA EGGS |
title_full | THYMINE IN THE ACID-SOLUBLE FRACTION OF ARBACIA EGGS |
title_fullStr | THYMINE IN THE ACID-SOLUBLE FRACTION OF ARBACIA EGGS |
title_full_unstemmed | THYMINE IN THE ACID-SOLUBLE FRACTION OF ARBACIA EGGS |
title_short | THYMINE IN THE ACID-SOLUBLE FRACTION OF ARBACIA EGGS |
title_sort | thymine in the acid-soluble fraction of arbacia eggs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223772/ https://www.ncbi.nlm.nih.gov/pubmed/14381439 |
work_keys_str_mv | AT marshaka thymineintheacidsolublefractionofarbaciaeggs AT marshakc thymineintheacidsolublefractionofarbaciaeggs |