Reversion of multidrug resistance with polyalkylcyanoacrylate nanoparticles: towards a mechanism of action.

Polyalkylcyanoacrylate (PACA) nanoparticles loaded with doxorubicin allowed multidrug resistance to be overcome in vitro. However, increased cytotoxicity is not always correlated with an increased level of intracellular drug. Although we have previously shown that PACA nanoparticles are not endocyto...

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Autores principales: de Verdière, A. C., Dubernet, C., Némati, F., Soma, E., Appel, M., Ferté, J., Bernard, S., Puisieux, F., Couvreur, P.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1997
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223924/
https://www.ncbi.nlm.nih.gov/pubmed/9231919
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author de Verdière, A. C.
Dubernet, C.
Némati, F.
Soma, E.
Appel, M.
Ferté, J.
Bernard, S.
Puisieux, F.
Couvreur, P.
author_facet de Verdière, A. C.
Dubernet, C.
Némati, F.
Soma, E.
Appel, M.
Ferté, J.
Bernard, S.
Puisieux, F.
Couvreur, P.
author_sort de Verdière, A. C.
collection PubMed
description Polyalkylcyanoacrylate (PACA) nanoparticles loaded with doxorubicin allowed multidrug resistance to be overcome in vitro. However, increased cytotoxicity is not always correlated with an increased level of intracellular drug. Although we have previously shown that PACA nanoparticles are not endocytosed by tumour cells, we report here that a direct interaction between nanoparticles and cells is a necessary requirement for overcoming resistance. In addition, the results showed that the degradation products of PACA (mainly polycyanoacrylic acid) in the presence of doxorubicin are able to increase both accumulation and cytotoxicity, thus suggesting the formation of a doxorubicin-polycyanoacrylic acid ion pair. It is therefore concluded that resistance is overcome as a result of both the adsorption of nanoparticles to the cell surface and increased doxorubicin diffusion by the accumulation of an ion pair at the plasma membrane.
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spelling pubmed-22239242009-09-10 Reversion of multidrug resistance with polyalkylcyanoacrylate nanoparticles: towards a mechanism of action. de Verdière, A. C. Dubernet, C. Némati, F. Soma, E. Appel, M. Ferté, J. Bernard, S. Puisieux, F. Couvreur, P. Br J Cancer Research Article Polyalkylcyanoacrylate (PACA) nanoparticles loaded with doxorubicin allowed multidrug resistance to be overcome in vitro. However, increased cytotoxicity is not always correlated with an increased level of intracellular drug. Although we have previously shown that PACA nanoparticles are not endocytosed by tumour cells, we report here that a direct interaction between nanoparticles and cells is a necessary requirement for overcoming resistance. In addition, the results showed that the degradation products of PACA (mainly polycyanoacrylic acid) in the presence of doxorubicin are able to increase both accumulation and cytotoxicity, thus suggesting the formation of a doxorubicin-polycyanoacrylic acid ion pair. It is therefore concluded that resistance is overcome as a result of both the adsorption of nanoparticles to the cell surface and increased doxorubicin diffusion by the accumulation of an ion pair at the plasma membrane. Nature Publishing Group 1997 /pmc/articles/PMC2223924/ /pubmed/9231919 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
de Verdière, A. C.
Dubernet, C.
Némati, F.
Soma, E.
Appel, M.
Ferté, J.
Bernard, S.
Puisieux, F.
Couvreur, P.
Reversion of multidrug resistance with polyalkylcyanoacrylate nanoparticles: towards a mechanism of action.
title Reversion of multidrug resistance with polyalkylcyanoacrylate nanoparticles: towards a mechanism of action.
title_full Reversion of multidrug resistance with polyalkylcyanoacrylate nanoparticles: towards a mechanism of action.
title_fullStr Reversion of multidrug resistance with polyalkylcyanoacrylate nanoparticles: towards a mechanism of action.
title_full_unstemmed Reversion of multidrug resistance with polyalkylcyanoacrylate nanoparticles: towards a mechanism of action.
title_short Reversion of multidrug resistance with polyalkylcyanoacrylate nanoparticles: towards a mechanism of action.
title_sort reversion of multidrug resistance with polyalkylcyanoacrylate nanoparticles: towards a mechanism of action.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223924/
https://www.ncbi.nlm.nih.gov/pubmed/9231919
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