A COMPARISON OF NUCLEAR DRY WEIGHTS DETERMINED BY CHEMICAL AND BY INTERFEROMETRIC METHODS

1. The nuclei of cells from the thymus of the calf were isolated by three different techniques; the citric acid, the sucrose-calcium chloride, and the non-aqueous. 2. The mean dry weights of the nuclei were determined by chemical methods and by microscopic interferometry. There was a close correlati...

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Detalles Bibliográficos
Autores principales: Hale, Arthur J., Kay, Ernest R. M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1956
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223962/
https://www.ncbi.nlm.nih.gov/pubmed/13319378
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author Hale, Arthur J.
Kay, Ernest R. M.
author_facet Hale, Arthur J.
Kay, Ernest R. M.
author_sort Hale, Arthur J.
collection PubMed
description 1. The nuclei of cells from the thymus of the calf were isolated by three different techniques; the citric acid, the sucrose-calcium chloride, and the non-aqueous. 2. The mean dry weights of the nuclei were determined by chemical methods and by microscopic interferometry. There was a close correlation between the results from the interferometric and chemical methods. 3. The range of values about that mean was determined in each sample: the nuclei isolated in aqueous media contained approximately 45 per cent less material than those isolated in non-aqueous media. 4. The variations in dry weight with varying nuclear type are discussed. 5. The possible relationship between DNA content and dry weight is discussed.
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spelling pubmed-22239622008-05-01 A COMPARISON OF NUCLEAR DRY WEIGHTS DETERMINED BY CHEMICAL AND BY INTERFEROMETRIC METHODS Hale, Arthur J. Kay, Ernest R. M. J Biophys Biochem Cytol Article 1. The nuclei of cells from the thymus of the calf were isolated by three different techniques; the citric acid, the sucrose-calcium chloride, and the non-aqueous. 2. The mean dry weights of the nuclei were determined by chemical methods and by microscopic interferometry. There was a close correlation between the results from the interferometric and chemical methods. 3. The range of values about that mean was determined in each sample: the nuclei isolated in aqueous media contained approximately 45 per cent less material than those isolated in non-aqueous media. 4. The variations in dry weight with varying nuclear type are discussed. 5. The possible relationship between DNA content and dry weight is discussed. The Rockefeller University Press 1956-03-25 /pmc/articles/PMC2223962/ /pubmed/13319378 Text en Copyright © Copyright, 1956, by The Rockefeller Institute for Medical Research
spellingShingle Article
Hale, Arthur J.
Kay, Ernest R. M.
A COMPARISON OF NUCLEAR DRY WEIGHTS DETERMINED BY CHEMICAL AND BY INTERFEROMETRIC METHODS
title A COMPARISON OF NUCLEAR DRY WEIGHTS DETERMINED BY CHEMICAL AND BY INTERFEROMETRIC METHODS
title_full A COMPARISON OF NUCLEAR DRY WEIGHTS DETERMINED BY CHEMICAL AND BY INTERFEROMETRIC METHODS
title_fullStr A COMPARISON OF NUCLEAR DRY WEIGHTS DETERMINED BY CHEMICAL AND BY INTERFEROMETRIC METHODS
title_full_unstemmed A COMPARISON OF NUCLEAR DRY WEIGHTS DETERMINED BY CHEMICAL AND BY INTERFEROMETRIC METHODS
title_short A COMPARISON OF NUCLEAR DRY WEIGHTS DETERMINED BY CHEMICAL AND BY INTERFEROMETRIC METHODS
title_sort comparison of nuclear dry weights determined by chemical and by interferometric methods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223962/
https://www.ncbi.nlm.nih.gov/pubmed/13319378
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