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Polarization Optical Properties of the Pancreatic Acinar Cell of the Mouse

The birefringence of fresh and fixed mouse pancreatic acinar tissue was studied, utilizing whole mounts of pancreas from which the mesentery had been removed. Fresh pancreas in Tyrode's solution demonstrated positive birefringence with respect to the radial axis (the axis radiating from the nuc...

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Autor principal: Munger, Bryce L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1958
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224338/
https://www.ncbi.nlm.nih.gov/pubmed/13525431
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author Munger, Bryce L.
author_facet Munger, Bryce L.
author_sort Munger, Bryce L.
collection PubMed
description The birefringence of fresh and fixed mouse pancreatic acinar tissue was studied, utilizing whole mounts of pancreas from which the mesentery had been removed. Fresh pancreas in Tyrode's solution demonstrated positive birefringence with respect to the radial axis (the axis radiating from the nucleus as spokes from a wheel). Formol fixation reversed the sign of birefringence to negative with respect to the radius. The magnitude of birefringence increased with longer fixation. Neutral formol also reversed the sign to radially negative, but the magnitude did not increase with longer fixation. Tissue fixed in 2 per cent osmium tetroxide or potassium permanganate demonstrated strongly negative birefringence with respect to the radius. The cytoplasm of tissue fixed in acetic acid, though finely granular, still possessed radially positive birefringence. Birefringent areas could be seen in tissue fixed in potassium dichromate, but the sign of birefringence could not be determined. Ethyl alcohol, chromic acid, picric acid, and mercuric chloride all produced a brilliant cytoplasm in which no birefringence could be demonstrated. Freezing markedly decreased the radially positive birefringence of fresh tissue. Fresh tissue placed in increasing concentrations of glycerol demonstrated increasing radially positive birefringence. When formol-fixed tissue was placed in glycerol, the radially negative birefringence decreased. Osmium tetroxide-fixed tissue in 50 per cent glycerol was isotropic. The granularity of ethyl alcohol-fixed tissue disappeared in 50 per cent glycerol, and radially positive birefringence was evident. Frozen tissue showed increasing radially positive birefringence in increasing concentrations of glycerol. The results are discussed in relation to theories of fixation.
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spelling pubmed-22243382008-05-01 Polarization Optical Properties of the Pancreatic Acinar Cell of the Mouse Munger, Bryce L. J Biophys Biochem Cytol Article The birefringence of fresh and fixed mouse pancreatic acinar tissue was studied, utilizing whole mounts of pancreas from which the mesentery had been removed. Fresh pancreas in Tyrode's solution demonstrated positive birefringence with respect to the radial axis (the axis radiating from the nucleus as spokes from a wheel). Formol fixation reversed the sign of birefringence to negative with respect to the radius. The magnitude of birefringence increased with longer fixation. Neutral formol also reversed the sign to radially negative, but the magnitude did not increase with longer fixation. Tissue fixed in 2 per cent osmium tetroxide or potassium permanganate demonstrated strongly negative birefringence with respect to the radius. The cytoplasm of tissue fixed in acetic acid, though finely granular, still possessed radially positive birefringence. Birefringent areas could be seen in tissue fixed in potassium dichromate, but the sign of birefringence could not be determined. Ethyl alcohol, chromic acid, picric acid, and mercuric chloride all produced a brilliant cytoplasm in which no birefringence could be demonstrated. Freezing markedly decreased the radially positive birefringence of fresh tissue. Fresh tissue placed in increasing concentrations of glycerol demonstrated increasing radially positive birefringence. When formol-fixed tissue was placed in glycerol, the radially negative birefringence decreased. Osmium tetroxide-fixed tissue in 50 per cent glycerol was isotropic. The granularity of ethyl alcohol-fixed tissue disappeared in 50 per cent glycerol, and radially positive birefringence was evident. Frozen tissue showed increasing radially positive birefringence in increasing concentrations of glycerol. The results are discussed in relation to theories of fixation. The Rockefeller University Press 1958-03-25 /pmc/articles/PMC2224338/ /pubmed/13525431 Text en Copyright © Copyright, 1958, by The Rockefeller Institute
spellingShingle Article
Munger, Bryce L.
Polarization Optical Properties of the Pancreatic Acinar Cell of the Mouse
title Polarization Optical Properties of the Pancreatic Acinar Cell of the Mouse
title_full Polarization Optical Properties of the Pancreatic Acinar Cell of the Mouse
title_fullStr Polarization Optical Properties of the Pancreatic Acinar Cell of the Mouse
title_full_unstemmed Polarization Optical Properties of the Pancreatic Acinar Cell of the Mouse
title_short Polarization Optical Properties of the Pancreatic Acinar Cell of the Mouse
title_sort polarization optical properties of the pancreatic acinar cell of the mouse
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224338/
https://www.ncbi.nlm.nih.gov/pubmed/13525431
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