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Araldite as an Embedding Medium for Electron Microscopy

Epoxy resins are suitable media for embedding for electron microscopy, as they set uniformly with virtually no shrinkage. A mixture of araldite epoxy resins has been developed which is soluble in ethanol, and which yields a block of the required hardness for thin sectioning. The critical modificatio...

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Detalles Bibliográficos
Autores principales: Glauert, Audrey M., Glauert, R. H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1958
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224339/
https://www.ncbi.nlm.nih.gov/pubmed/13525433
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author Glauert, Audrey M.
Glauert, R. H.
author_facet Glauert, Audrey M.
Glauert, R. H.
author_sort Glauert, Audrey M.
collection PubMed
description Epoxy resins are suitable media for embedding for electron microscopy, as they set uniformly with virtually no shrinkage. A mixture of araldite epoxy resins has been developed which is soluble in ethanol, and which yields a block of the required hardness for thin sectioning. The critical modifications to the conventional mixtures are the choice of a plasticized resin in conjunction with an aliphatic anhydride as the hardener. The hardness of the final block can be varied by incorporating additional plasticizer, and the rate of setting can be controlled by the use of an amine accelerator. The properties of the araldite mixture can be varied quite widely by adjusting the proportions of the various constituents. The procedure for embedding biological specimens is similar to that employed with methacrylates, although longer soaking times are recommended to ensure the complete penetration of the more viscous epoxy resin. An improvement in the preservation of the fine structure of a variety of specimens has already been reported, and a typical electron microgram illustrates the present paper.
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spelling pubmed-22243392008-05-01 Araldite as an Embedding Medium for Electron Microscopy Glauert, Audrey M. Glauert, R. H. J Biophys Biochem Cytol Article Epoxy resins are suitable media for embedding for electron microscopy, as they set uniformly with virtually no shrinkage. A mixture of araldite epoxy resins has been developed which is soluble in ethanol, and which yields a block of the required hardness for thin sectioning. The critical modifications to the conventional mixtures are the choice of a plasticized resin in conjunction with an aliphatic anhydride as the hardener. The hardness of the final block can be varied by incorporating additional plasticizer, and the rate of setting can be controlled by the use of an amine accelerator. The properties of the araldite mixture can be varied quite widely by adjusting the proportions of the various constituents. The procedure for embedding biological specimens is similar to that employed with methacrylates, although longer soaking times are recommended to ensure the complete penetration of the more viscous epoxy resin. An improvement in the preservation of the fine structure of a variety of specimens has already been reported, and a typical electron microgram illustrates the present paper. The Rockefeller University Press 1958-03-25 /pmc/articles/PMC2224339/ /pubmed/13525433 Text en Copyright © Copyright, 1958, by The Rockefeller Institute
spellingShingle Article
Glauert, Audrey M.
Glauert, R. H.
Araldite as an Embedding Medium for Electron Microscopy
title Araldite as an Embedding Medium for Electron Microscopy
title_full Araldite as an Embedding Medium for Electron Microscopy
title_fullStr Araldite as an Embedding Medium for Electron Microscopy
title_full_unstemmed Araldite as an Embedding Medium for Electron Microscopy
title_short Araldite as an Embedding Medium for Electron Microscopy
title_sort araldite as an embedding medium for electron microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224339/
https://www.ncbi.nlm.nih.gov/pubmed/13525433
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