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Structural Alterations in Nerve Fibers Produced by Hypotonic and Hypertonic Solutions

In sections of KMnO(4)-fixed, developing mouse sciatic nerves, the central gap of mesaxons in myelinating fibers is normally closed with close apposition of the outside ∼20 A dense strata of the two ∼75 A Schwann cell membranes. The two combined outside strata make the intraperiod line bisecting eac...

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Detalles Bibliográficos
Autor principal: Robertson, J. David
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1958
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224500/
https://www.ncbi.nlm.nih.gov/pubmed/13563541
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author Robertson, J. David
author_facet Robertson, J. David
author_sort Robertson, J. David
collection PubMed
description In sections of KMnO(4)-fixed, developing mouse sciatic nerves, the central gap of mesaxons in myelinating fibers is normally closed with close apposition of the outside ∼20 A dense strata of the two ∼75 A Schwann cell membranes. The two combined outside strata make the intraperiod line bisecting each myelin lamella. The ∼150 A mesaxon is elaborated spirally around the axon in either a right hand or left hand spiral, and its inside (cytoplasmic) ∼20 A strata in apposition form the major dense lines of myelin. In hypotonic solutions the lamellae of adult frog sciatic myelinated fibers split apart along the outside membrane strata apposed at the intraperiod line throughout the spiral. Under similar conditions the inside (cytoplasmic) strata of the membranes, in apposition at the major dense lines, do not separate. The ∼150 A membranous structure resulting from this is called an "internal compound membrane." The double membranes of normal and control frog sciatic unmyelinated fibers have a central gap ∼100 to 150 A wide. After soaking in 4 to 10 times normal strength Ringer solution or 10 N sucrose-Ringer solution, this gap closes and a membranous structure ∼150 A wide resembling developing mouse mesaxons results. This is designated by the term "external compound membrane." The latter membranes resemble internal compound membranes, but their central dense zones, each consisting of two apposed outside membrane strata, are less dense.
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spelling pubmed-22245002008-05-01 Structural Alterations in Nerve Fibers Produced by Hypotonic and Hypertonic Solutions Robertson, J. David J Biophys Biochem Cytol Article In sections of KMnO(4)-fixed, developing mouse sciatic nerves, the central gap of mesaxons in myelinating fibers is normally closed with close apposition of the outside ∼20 A dense strata of the two ∼75 A Schwann cell membranes. The two combined outside strata make the intraperiod line bisecting each myelin lamella. The ∼150 A mesaxon is elaborated spirally around the axon in either a right hand or left hand spiral, and its inside (cytoplasmic) ∼20 A strata in apposition form the major dense lines of myelin. In hypotonic solutions the lamellae of adult frog sciatic myelinated fibers split apart along the outside membrane strata apposed at the intraperiod line throughout the spiral. Under similar conditions the inside (cytoplasmic) strata of the membranes, in apposition at the major dense lines, do not separate. The ∼150 A membranous structure resulting from this is called an "internal compound membrane." The double membranes of normal and control frog sciatic unmyelinated fibers have a central gap ∼100 to 150 A wide. After soaking in 4 to 10 times normal strength Ringer solution or 10 N sucrose-Ringer solution, this gap closes and a membranous structure ∼150 A wide resembling developing mouse mesaxons results. This is designated by the term "external compound membrane." The latter membranes resemble internal compound membranes, but their central dense zones, each consisting of two apposed outside membrane strata, are less dense. The Rockefeller University Press 1958-07-25 /pmc/articles/PMC2224500/ /pubmed/13563541 Text en Copyright © Copyright, 1958, by The Rockefeller Institute
spellingShingle Article
Robertson, J. David
Structural Alterations in Nerve Fibers Produced by Hypotonic and Hypertonic Solutions
title Structural Alterations in Nerve Fibers Produced by Hypotonic and Hypertonic Solutions
title_full Structural Alterations in Nerve Fibers Produced by Hypotonic and Hypertonic Solutions
title_fullStr Structural Alterations in Nerve Fibers Produced by Hypotonic and Hypertonic Solutions
title_full_unstemmed Structural Alterations in Nerve Fibers Produced by Hypotonic and Hypertonic Solutions
title_short Structural Alterations in Nerve Fibers Produced by Hypotonic and Hypertonic Solutions
title_sort structural alterations in nerve fibers produced by hypotonic and hypertonic solutions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224500/
https://www.ncbi.nlm.nih.gov/pubmed/13563541
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