Intracellular Distribution of 5' Nucleotidase in Rat Liver

The intracellular distribution of 5' nucleotidase was investigated in rat liver by biochemical analysis of cell fractions obtained by differential centrifugation. The enzymatic activity was measured by determination of the inorganic phosphorus liberated from 5' nucleotides. The 5' nuc...

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Detalles Bibliográficos
Autores principales: de Lamirande, Gaston, Allard, Claude, Cantero, Antonio
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1958
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224501/
https://www.ncbi.nlm.nih.gov/pubmed/13563543
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author de Lamirande, Gaston
Allard, Claude
Cantero, Antonio
author_facet de Lamirande, Gaston
Allard, Claude
Cantero, Antonio
author_sort de Lamirande, Gaston
collection PubMed
description The intracellular distribution of 5' nucleotidase was investigated in rat liver by biochemical analysis of cell fractions obtained by differential centrifugation. The enzymatic activity was measured by determination of the inorganic phosphorus liberated from 5' nucleotides. The 5' nucleotidase activity was mainly found in the nuclear and microsomal fractions. An attempt to extract the enzyme from these fractions with Mg(++) ion solutions was unsuccessful. It is concluded that 5' nucleotidase is actually present in the nuclear and microsomal fractions of rat liver cells.
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spelling pubmed-22245012008-05-01 Intracellular Distribution of 5' Nucleotidase in Rat Liver de Lamirande, Gaston Allard, Claude Cantero, Antonio J Biophys Biochem Cytol Article The intracellular distribution of 5' nucleotidase was investigated in rat liver by biochemical analysis of cell fractions obtained by differential centrifugation. The enzymatic activity was measured by determination of the inorganic phosphorus liberated from 5' nucleotides. The 5' nucleotidase activity was mainly found in the nuclear and microsomal fractions. An attempt to extract the enzyme from these fractions with Mg(++) ion solutions was unsuccessful. It is concluded that 5' nucleotidase is actually present in the nuclear and microsomal fractions of rat liver cells. The Rockefeller University Press 1958-07-25 /pmc/articles/PMC2224501/ /pubmed/13563543 Text en Copyright © Copyright, 1958, by The Rockefeller Institute
spellingShingle Article
de Lamirande, Gaston
Allard, Claude
Cantero, Antonio
Intracellular Distribution of 5' Nucleotidase in Rat Liver
title Intracellular Distribution of 5' Nucleotidase in Rat Liver
title_full Intracellular Distribution of 5' Nucleotidase in Rat Liver
title_fullStr Intracellular Distribution of 5' Nucleotidase in Rat Liver
title_full_unstemmed Intracellular Distribution of 5' Nucleotidase in Rat Liver
title_short Intracellular Distribution of 5' Nucleotidase in Rat Liver
title_sort intracellular distribution of 5' nucleotidase in rat liver
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224501/
https://www.ncbi.nlm.nih.gov/pubmed/13563543
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AT allardclaude intracellulardistributionof5nucleotidaseinratliver
AT canteroantonio intracellulardistributionof5nucleotidaseinratliver

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