The Two-Wavelength Method of Microspectrophotometry : II. A Set of Tables to Facilitate the Calculations

The calculations required for two-wavelength measurements are time consuming and laborious. In order to circumvent this limitation of the method, a set of tables which combined four operations into one has been designed and is reproduced within. The tables are based on Patau's formulae. The two...

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Detalles Bibliográficos
Autor principal: Mendelsohn, Mortimer L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1958
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224502/
https://www.ncbi.nlm.nih.gov/pubmed/13563549
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author Mendelsohn, Mortimer L.
author_facet Mendelsohn, Mortimer L.
author_sort Mendelsohn, Mortimer L.
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description The calculations required for two-wavelength measurements are time consuming and laborious. In order to circumvent this limitation of the method, a set of tables which combined four operations into one has been designed and is reproduced within. The tables are based on Patau's formulae. The two transmission readings obtained according to the photometric method provide the coordinates which lead directly to a value for the relative absorbance. The product of this absorbance and the area of the photometric field gives the relative amount of chromophore in the field. The range of transmission values covered in the table corresponds to the effective range of the two-wavelength method.
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spelling pubmed-22245022008-05-01 The Two-Wavelength Method of Microspectrophotometry : II. A Set of Tables to Facilitate the Calculations Mendelsohn, Mortimer L. J Biophys Biochem Cytol Article The calculations required for two-wavelength measurements are time consuming and laborious. In order to circumvent this limitation of the method, a set of tables which combined four operations into one has been designed and is reproduced within. The tables are based on Patau's formulae. The two transmission readings obtained according to the photometric method provide the coordinates which lead directly to a value for the relative absorbance. The product of this absorbance and the area of the photometric field gives the relative amount of chromophore in the field. The range of transmission values covered in the table corresponds to the effective range of the two-wavelength method. The Rockefeller University Press 1958-07-25 /pmc/articles/PMC2224502/ /pubmed/13563549 Text en Copyright © Copyright, 1958, by The Rockefeller Institute
spellingShingle Article
Mendelsohn, Mortimer L.
The Two-Wavelength Method of Microspectrophotometry : II. A Set of Tables to Facilitate the Calculations
title The Two-Wavelength Method of Microspectrophotometry : II. A Set of Tables to Facilitate the Calculations
title_full The Two-Wavelength Method of Microspectrophotometry : II. A Set of Tables to Facilitate the Calculations
title_fullStr The Two-Wavelength Method of Microspectrophotometry : II. A Set of Tables to Facilitate the Calculations
title_full_unstemmed The Two-Wavelength Method of Microspectrophotometry : II. A Set of Tables to Facilitate the Calculations
title_short The Two-Wavelength Method of Microspectrophotometry : II. A Set of Tables to Facilitate the Calculations
title_sort two-wavelength method of microspectrophotometry : ii. a set of tables to facilitate the calculations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224502/
https://www.ncbi.nlm.nih.gov/pubmed/13563549
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