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Adenosinetriphosphatase Activity in the Cell Membranes of Kidney Tubule Cells

This cytochemical study demonstrates high levels of apparent ATPase activity in the infolded cell membranes of the proximal tubules (dog, rat, human, mouse, monkey, and opossum) and ascending loops of Henle (dog, rat, human and, to a variable degree, mouse). Electron microscopy has shown (see Rhodin...

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Detalles Bibliográficos
Autores principales: Spater, Herman W., Novikoff, Alex B., Masek, Bertha
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1958
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224524/
https://www.ncbi.nlm.nih.gov/pubmed/13610942
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author Spater, Herman W.
Novikoff, Alex B.
Masek, Bertha
author_facet Spater, Herman W.
Novikoff, Alex B.
Masek, Bertha
author_sort Spater, Herman W.
collection PubMed
description This cytochemical study demonstrates high levels of apparent ATPase activity in the infolded cell membranes of the proximal tubules (dog, rat, human, mouse, monkey, and opossum) and ascending loops of Henle (dog, rat, human and, to a variable degree, mouse). Electron microscopy has shown (see Rhodin (1)) that these membranes separate adjacent cells where they interlock with one another by multiple cytoplasmic lamellae containing oriented mitochondria. The significance of the high ATPase activity is considered in relation to possible movements of the membranes and to "active transport" believed to occur there. In the rat, enzyme activity in the proximal tubule membranes does not survive formol-calcium fixation, and it is therefore necessary to use unfixed sections for its demonstration. However, in edematous rats with experimental nephrosis (induced by the injection of aminonucleoside or with antikidney serum) marked ATPase activity is exhibited in these membranes even after formol-calcium fixation. When proximal tubule or Henle loop cells of the dog acquire an altered metabolism, as indicated by accumulated lipide spheres or by "droplets," the infolded ATPase-rich membranes are no longer demonstrable.
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spelling pubmed-22245242008-05-01 Adenosinetriphosphatase Activity in the Cell Membranes of Kidney Tubule Cells Spater, Herman W. Novikoff, Alex B. Masek, Bertha J Biophys Biochem Cytol Article This cytochemical study demonstrates high levels of apparent ATPase activity in the infolded cell membranes of the proximal tubules (dog, rat, human, mouse, monkey, and opossum) and ascending loops of Henle (dog, rat, human and, to a variable degree, mouse). Electron microscopy has shown (see Rhodin (1)) that these membranes separate adjacent cells where they interlock with one another by multiple cytoplasmic lamellae containing oriented mitochondria. The significance of the high ATPase activity is considered in relation to possible movements of the membranes and to "active transport" believed to occur there. In the rat, enzyme activity in the proximal tubule membranes does not survive formol-calcium fixation, and it is therefore necessary to use unfixed sections for its demonstration. However, in edematous rats with experimental nephrosis (induced by the injection of aminonucleoside or with antikidney serum) marked ATPase activity is exhibited in these membranes even after formol-calcium fixation. When proximal tubule or Henle loop cells of the dog acquire an altered metabolism, as indicated by accumulated lipide spheres or by "droplets," the infolded ATPase-rich membranes are no longer demonstrable. The Rockefeller University Press 1958-11-25 /pmc/articles/PMC2224524/ /pubmed/13610942 Text en Copyright © Copyright, 1958, by The Rockefeller Institute
spellingShingle Article
Spater, Herman W.
Novikoff, Alex B.
Masek, Bertha
Adenosinetriphosphatase Activity in the Cell Membranes of Kidney Tubule Cells
title Adenosinetriphosphatase Activity in the Cell Membranes of Kidney Tubule Cells
title_full Adenosinetriphosphatase Activity in the Cell Membranes of Kidney Tubule Cells
title_fullStr Adenosinetriphosphatase Activity in the Cell Membranes of Kidney Tubule Cells
title_full_unstemmed Adenosinetriphosphatase Activity in the Cell Membranes of Kidney Tubule Cells
title_short Adenosinetriphosphatase Activity in the Cell Membranes of Kidney Tubule Cells
title_sort adenosinetriphosphatase activity in the cell membranes of kidney tubule cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224524/
https://www.ncbi.nlm.nih.gov/pubmed/13610942
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