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A Cytochemical Study on the Pancreas of the Guinea Pig : III. In Vivo Incorporation of Leucine-1-C(14) into the Proteins of Cell Fractions

DL-leucine-1-C(14) was administered by intracardiac injection to guinea pigs and its in vivo incorporation into the proteins of various pancreatic cell fractions followed over a period of 2 hours. The pancreas was homogenized in 0.88 M sucrose and fractionated by differential centrifugation to give...

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Detalles Bibliográficos
Autores principales: Siekevitz, Philip, Palade, George E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1958
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224556/
https://www.ncbi.nlm.nih.gov/pubmed/13587549
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author Siekevitz, Philip
Palade, George E.
author_facet Siekevitz, Philip
Palade, George E.
author_sort Siekevitz, Philip
collection PubMed
description DL-leucine-1-C(14) was administered by intracardiac injection to guinea pigs and its in vivo incorporation into the proteins of various pancreatic cell fractions followed over a period of 2 hours. The pancreas was homogenized in 0.88 M sucrose and fractionated by differential centrifugation to give nuclear, zymogen, mitochondrial, microsomal, postmicrosomal, and final supernatant fractions. The proteins of these fractions, obtained by precipitation with trichloroacetic acid followed by washing, were counted. The proteins of the microsomal fraction showed the highest early specific activity and were followed by those of the zymogen and mitochondrial fractions. The microsomal fraction was broken up into two subfractions: one consisting of detached RNP particles, the other representing mainly the microsomal content and membranes. The incorporation of labelled leucine into the proteins of microsomal subtractions and in those of postmicrosomal fractions was studied comparatively in the pancreas of fasted and fed guinea pigs as well as in the liver and pancreas of fasted animals. A tentative cytological picture of protein synthesis and transport based on these findings is presented.
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spelling pubmed-22245562008-05-01 A Cytochemical Study on the Pancreas of the Guinea Pig : III. In Vivo Incorporation of Leucine-1-C(14) into the Proteins of Cell Fractions Siekevitz, Philip Palade, George E. J Biophys Biochem Cytol Article DL-leucine-1-C(14) was administered by intracardiac injection to guinea pigs and its in vivo incorporation into the proteins of various pancreatic cell fractions followed over a period of 2 hours. The pancreas was homogenized in 0.88 M sucrose and fractionated by differential centrifugation to give nuclear, zymogen, mitochondrial, microsomal, postmicrosomal, and final supernatant fractions. The proteins of these fractions, obtained by precipitation with trichloroacetic acid followed by washing, were counted. The proteins of the microsomal fraction showed the highest early specific activity and were followed by those of the zymogen and mitochondrial fractions. The microsomal fraction was broken up into two subfractions: one consisting of detached RNP particles, the other representing mainly the microsomal content and membranes. The incorporation of labelled leucine into the proteins of microsomal subtractions and in those of postmicrosomal fractions was studied comparatively in the pancreas of fasted and fed guinea pigs as well as in the liver and pancreas of fasted animals. A tentative cytological picture of protein synthesis and transport based on these findings is presented. The Rockefeller University Press 1958-09-25 /pmc/articles/PMC2224556/ /pubmed/13587549 Text en Copyright © Copyright, 1958, by The Rockefeller Institute
spellingShingle Article
Siekevitz, Philip
Palade, George E.
A Cytochemical Study on the Pancreas of the Guinea Pig : III. In Vivo Incorporation of Leucine-1-C(14) into the Proteins of Cell Fractions
title A Cytochemical Study on the Pancreas of the Guinea Pig : III. In Vivo Incorporation of Leucine-1-C(14) into the Proteins of Cell Fractions
title_full A Cytochemical Study on the Pancreas of the Guinea Pig : III. In Vivo Incorporation of Leucine-1-C(14) into the Proteins of Cell Fractions
title_fullStr A Cytochemical Study on the Pancreas of the Guinea Pig : III. In Vivo Incorporation of Leucine-1-C(14) into the Proteins of Cell Fractions
title_full_unstemmed A Cytochemical Study on the Pancreas of the Guinea Pig : III. In Vivo Incorporation of Leucine-1-C(14) into the Proteins of Cell Fractions
title_short A Cytochemical Study on the Pancreas of the Guinea Pig : III. In Vivo Incorporation of Leucine-1-C(14) into the Proteins of Cell Fractions
title_sort cytochemical study on the pancreas of the guinea pig : iii. in vivo incorporation of leucine-1-c(14) into the proteins of cell fractions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224556/
https://www.ncbi.nlm.nih.gov/pubmed/13587549
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