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Mitochondrial Swelling Induced by Glutathione

Reduced glutathione, in concentrations approximating those occurring in intact rat liver, causes swelling of rat liver mitochondria in vitro which is different in kinetics and extent from that yielded by L-thyroxine. The effect is also given by cysteine, which is more active, and reduced coenzyme A,...

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Autores principales: Lehninger, Albert L., Schneider, Marion
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1959
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224615/
https://www.ncbi.nlm.nih.gov/pubmed/13630941
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author Lehninger, Albert L.
Schneider, Marion
author_facet Lehninger, Albert L.
Schneider, Marion
author_sort Lehninger, Albert L.
collection PubMed
description Reduced glutathione, in concentrations approximating those occurring in intact rat liver, causes swelling of rat liver mitochondria in vitro which is different in kinetics and extent from that yielded by L-thyroxine. The effect is also given by cysteine, which is more active, and reduced coenzyme A, but not by L-ascorbate, cystine, or oxidized glutathione. The optimum pH is 6.5, whereas thyroxine-induced swelling is optimal at pH 7.5. The GSH-induced swelling is not inhibited by DNP or dicumarol, nor by high concentrations of sucrose, serum albumin, or polyvinylpyrrolidone, in contrast to thyroxine-induced swelling. ATP inhibits the GSH swelling, but ADP and AMP are ineffective. Mn(-+) is a very potent inhibitor, but Mg(++) is ineffective. Ethylenediaminetetraacetate is also an effective inhibitor of GSH-induced swelling. The respiratory inhibitors amytal and antimycin A do not inhibit the swelling action of GSH, but cyanide does; these findings are consistent with the view that the oxidation-reduction state of the respiratory chain between cytochrome c and oxygen is a determinant of GSH-induced swelling. Reversal of GSH-induced swelling by osmotic means or by ATP in KCl media could not be observed. Large losses of nucleotides and protein occur during the swelling by GSH, suggesting that the action is irreversible. The characteristically drastic swelling action of GSH could be prevented if L-thyroxine was also present in the medium.
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spelling pubmed-22246152008-05-01 Mitochondrial Swelling Induced by Glutathione Lehninger, Albert L. Schneider, Marion J Biophys Biochem Cytol Article Reduced glutathione, in concentrations approximating those occurring in intact rat liver, causes swelling of rat liver mitochondria in vitro which is different in kinetics and extent from that yielded by L-thyroxine. The effect is also given by cysteine, which is more active, and reduced coenzyme A, but not by L-ascorbate, cystine, or oxidized glutathione. The optimum pH is 6.5, whereas thyroxine-induced swelling is optimal at pH 7.5. The GSH-induced swelling is not inhibited by DNP or dicumarol, nor by high concentrations of sucrose, serum albumin, or polyvinylpyrrolidone, in contrast to thyroxine-induced swelling. ATP inhibits the GSH swelling, but ADP and AMP are ineffective. Mn(-+) is a very potent inhibitor, but Mg(++) is ineffective. Ethylenediaminetetraacetate is also an effective inhibitor of GSH-induced swelling. The respiratory inhibitors amytal and antimycin A do not inhibit the swelling action of GSH, but cyanide does; these findings are consistent with the view that the oxidation-reduction state of the respiratory chain between cytochrome c and oxygen is a determinant of GSH-induced swelling. Reversal of GSH-induced swelling by osmotic means or by ATP in KCl media could not be observed. Large losses of nucleotides and protein occur during the swelling by GSH, suggesting that the action is irreversible. The characteristically drastic swelling action of GSH could be prevented if L-thyroxine was also present in the medium. The Rockefeller University Press 1959-01-25 /pmc/articles/PMC2224615/ /pubmed/13630941 Text en Copyright © Copyright, 1959, by The Rockefeller Institute
spellingShingle Article
Lehninger, Albert L.
Schneider, Marion
Mitochondrial Swelling Induced by Glutathione
title Mitochondrial Swelling Induced by Glutathione
title_full Mitochondrial Swelling Induced by Glutathione
title_fullStr Mitochondrial Swelling Induced by Glutathione
title_full_unstemmed Mitochondrial Swelling Induced by Glutathione
title_short Mitochondrial Swelling Induced by Glutathione
title_sort mitochondrial swelling induced by glutathione
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224615/
https://www.ncbi.nlm.nih.gov/pubmed/13630941
work_keys_str_mv AT lehningeralbertl mitochondrialswellinginducedbyglutathione
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