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The Absence of Histone in the Bacterium Escherichia coli : I. Preparation and Analysis of Nucleoprotein Extract
The deoxyribonucleic acid (DNA) from Escherichia coli has been isolated as an extract containing about 50 per cent by weight protein. The protein component differs both in composition and chemical behaviour from histone which occurs in combination with the DNA in most cells of higher organisms. Alth...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1959
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224627/ https://www.ncbi.nlm.nih.gov/pubmed/13630933 |
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author | Zubay, G. Watson, M. R. |
author_facet | Zubay, G. Watson, M. R. |
author_sort | Zubay, G. |
collection | PubMed |
description | The deoxyribonucleic acid (DNA) from Escherichia coli has been isolated as an extract containing about 50 per cent by weight protein. The protein component differs both in composition and chemical behaviour from histone which occurs in combination with the DNA in most cells of higher organisms. Although this result suggests the absence of histone-like protein, it is not clear whether the bacterial protein found is naturally bound to the bacterial DNA in the cell or becomes attached to the DNA during the course of isolation. |
format | Text |
id | pubmed-2224627 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1959 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22246272008-05-01 The Absence of Histone in the Bacterium Escherichia coli : I. Preparation and Analysis of Nucleoprotein Extract Zubay, G. Watson, M. R. J Biophys Biochem Cytol Article The deoxyribonucleic acid (DNA) from Escherichia coli has been isolated as an extract containing about 50 per cent by weight protein. The protein component differs both in composition and chemical behaviour from histone which occurs in combination with the DNA in most cells of higher organisms. Although this result suggests the absence of histone-like protein, it is not clear whether the bacterial protein found is naturally bound to the bacterial DNA in the cell or becomes attached to the DNA during the course of isolation. The Rockefeller University Press 1959-01-25 /pmc/articles/PMC2224627/ /pubmed/13630933 Text en Copyright © Copyright, 1959, by The Rockefeller Institute |
spellingShingle | Article Zubay, G. Watson, M. R. The Absence of Histone in the Bacterium Escherichia coli : I. Preparation and Analysis of Nucleoprotein Extract |
title | The Absence of Histone in the Bacterium Escherichia coli : I. Preparation and Analysis of Nucleoprotein Extract |
title_full | The Absence of Histone in the Bacterium Escherichia coli : I. Preparation and Analysis of Nucleoprotein Extract |
title_fullStr | The Absence of Histone in the Bacterium Escherichia coli : I. Preparation and Analysis of Nucleoprotein Extract |
title_full_unstemmed | The Absence of Histone in the Bacterium Escherichia coli : I. Preparation and Analysis of Nucleoprotein Extract |
title_short | The Absence of Histone in the Bacterium Escherichia coli : I. Preparation and Analysis of Nucleoprotein Extract |
title_sort | absence of histone in the bacterium escherichia coli : i. preparation and analysis of nucleoprotein extract |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224627/ https://www.ncbi.nlm.nih.gov/pubmed/13630933 |
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