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The Metabolism of Chromosomal Ribonucleic Acid in Drosophila Salivary Glands and its Relation to Synthesis of Desoxyribonucleic Acid

Incorporation of adenine-8-C(14) into chromosomal nucleic acids of Drosophila salivary glands has been observed with the autoradiographic technique. RNA-C(14) and DNA-C(14) were detected as the fractions extractable by ribonuclease digestion and resistant to ribonuclease, respectively. Extractions w...

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Autores principales: McMaster-Kaye, Rachel, Taylor, J. Herbert
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1959
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224675/
https://www.ncbi.nlm.nih.gov/pubmed/13664687
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author McMaster-Kaye, Rachel
Taylor, J. Herbert
author_facet McMaster-Kaye, Rachel
Taylor, J. Herbert
author_sort McMaster-Kaye, Rachel
collection PubMed
description Incorporation of adenine-8-C(14) into chromosomal nucleic acids of Drosophila salivary glands has been observed with the autoradiographic technique. RNA-C(14) and DNA-C(14) were detected as the fractions extractable by ribonuclease digestion and resistant to ribonuclease, respectively. Extractions with desoxyribonuclease and acids were also used to identify the nucleic acids. Time-course curves were determined from grain counts. RNA-C(14) concentration reached a maximum in 2 hours, and decreased after the 4th hour. DNA-C(14) concentration reached its maximum within 8 hours, and showed no decreases during a 48-hour experiment. In the latter part of the period of observation, morphological differentiation of the gland occurred, the decrease in RNA-C(14) became very rapid, and a large increase in DNA-C(14) was observed. Marked decrease in RNA-C(14) and increase in DNA-C(14) were detectable in a few hours when isotope was administered shortly before visible differentiation of the gland. Measurements of nuclear size indicated no significant decreases in RNA-C(14) amount prior to the period of differentiation. During this later period, a large decrease in RNA-C(14) amount occurs suddenly, and the same amount of C(14) is added simultaneously to the DNA fraction, as expected if RNA-C(14) is utilized in the synthesis of DNA.
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spelling pubmed-22246752008-05-01 The Metabolism of Chromosomal Ribonucleic Acid in Drosophila Salivary Glands and its Relation to Synthesis of Desoxyribonucleic Acid McMaster-Kaye, Rachel Taylor, J. Herbert J Biophys Biochem Cytol Article Incorporation of adenine-8-C(14) into chromosomal nucleic acids of Drosophila salivary glands has been observed with the autoradiographic technique. RNA-C(14) and DNA-C(14) were detected as the fractions extractable by ribonuclease digestion and resistant to ribonuclease, respectively. Extractions with desoxyribonuclease and acids were also used to identify the nucleic acids. Time-course curves were determined from grain counts. RNA-C(14) concentration reached a maximum in 2 hours, and decreased after the 4th hour. DNA-C(14) concentration reached its maximum within 8 hours, and showed no decreases during a 48-hour experiment. In the latter part of the period of observation, morphological differentiation of the gland occurred, the decrease in RNA-C(14) became very rapid, and a large increase in DNA-C(14) was observed. Marked decrease in RNA-C(14) and increase in DNA-C(14) were detectable in a few hours when isotope was administered shortly before visible differentiation of the gland. Measurements of nuclear size indicated no significant decreases in RNA-C(14) amount prior to the period of differentiation. During this later period, a large decrease in RNA-C(14) amount occurs suddenly, and the same amount of C(14) is added simultaneously to the DNA fraction, as expected if RNA-C(14) is utilized in the synthesis of DNA. The Rockefeller University Press 1959-05-01 /pmc/articles/PMC2224675/ /pubmed/13664687 Text en Copyright © Copyright, 1959, by The Rockefeller Institute
spellingShingle Article
McMaster-Kaye, Rachel
Taylor, J. Herbert
The Metabolism of Chromosomal Ribonucleic Acid in Drosophila Salivary Glands and its Relation to Synthesis of Desoxyribonucleic Acid
title The Metabolism of Chromosomal Ribonucleic Acid in Drosophila Salivary Glands and its Relation to Synthesis of Desoxyribonucleic Acid
title_full The Metabolism of Chromosomal Ribonucleic Acid in Drosophila Salivary Glands and its Relation to Synthesis of Desoxyribonucleic Acid
title_fullStr The Metabolism of Chromosomal Ribonucleic Acid in Drosophila Salivary Glands and its Relation to Synthesis of Desoxyribonucleic Acid
title_full_unstemmed The Metabolism of Chromosomal Ribonucleic Acid in Drosophila Salivary Glands and its Relation to Synthesis of Desoxyribonucleic Acid
title_short The Metabolism of Chromosomal Ribonucleic Acid in Drosophila Salivary Glands and its Relation to Synthesis of Desoxyribonucleic Acid
title_sort metabolism of chromosomal ribonucleic acid in drosophila salivary glands and its relation to synthesis of desoxyribonucleic acid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224675/
https://www.ncbi.nlm.nih.gov/pubmed/13664687
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