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The Metabolism of Chromosomal Ribonucleic Acid in Drosophila Salivary Glands and its Relation to Synthesis of Desoxyribonucleic Acid
Incorporation of adenine-8-C(14) into chromosomal nucleic acids of Drosophila salivary glands has been observed with the autoradiographic technique. RNA-C(14) and DNA-C(14) were detected as the fractions extractable by ribonuclease digestion and resistant to ribonuclease, respectively. Extractions w...
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1959
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224675/ https://www.ncbi.nlm.nih.gov/pubmed/13664687 |
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author | McMaster-Kaye, Rachel Taylor, J. Herbert |
author_facet | McMaster-Kaye, Rachel Taylor, J. Herbert |
author_sort | McMaster-Kaye, Rachel |
collection | PubMed |
description | Incorporation of adenine-8-C(14) into chromosomal nucleic acids of Drosophila salivary glands has been observed with the autoradiographic technique. RNA-C(14) and DNA-C(14) were detected as the fractions extractable by ribonuclease digestion and resistant to ribonuclease, respectively. Extractions with desoxyribonuclease and acids were also used to identify the nucleic acids. Time-course curves were determined from grain counts. RNA-C(14) concentration reached a maximum in 2 hours, and decreased after the 4th hour. DNA-C(14) concentration reached its maximum within 8 hours, and showed no decreases during a 48-hour experiment. In the latter part of the period of observation, morphological differentiation of the gland occurred, the decrease in RNA-C(14) became very rapid, and a large increase in DNA-C(14) was observed. Marked decrease in RNA-C(14) and increase in DNA-C(14) were detectable in a few hours when isotope was administered shortly before visible differentiation of the gland. Measurements of nuclear size indicated no significant decreases in RNA-C(14) amount prior to the period of differentiation. During this later period, a large decrease in RNA-C(14) amount occurs suddenly, and the same amount of C(14) is added simultaneously to the DNA fraction, as expected if RNA-C(14) is utilized in the synthesis of DNA. |
format | Text |
id | pubmed-2224675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1959 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22246752008-05-01 The Metabolism of Chromosomal Ribonucleic Acid in Drosophila Salivary Glands and its Relation to Synthesis of Desoxyribonucleic Acid McMaster-Kaye, Rachel Taylor, J. Herbert J Biophys Biochem Cytol Article Incorporation of adenine-8-C(14) into chromosomal nucleic acids of Drosophila salivary glands has been observed with the autoradiographic technique. RNA-C(14) and DNA-C(14) were detected as the fractions extractable by ribonuclease digestion and resistant to ribonuclease, respectively. Extractions with desoxyribonuclease and acids were also used to identify the nucleic acids. Time-course curves were determined from grain counts. RNA-C(14) concentration reached a maximum in 2 hours, and decreased after the 4th hour. DNA-C(14) concentration reached its maximum within 8 hours, and showed no decreases during a 48-hour experiment. In the latter part of the period of observation, morphological differentiation of the gland occurred, the decrease in RNA-C(14) became very rapid, and a large increase in DNA-C(14) was observed. Marked decrease in RNA-C(14) and increase in DNA-C(14) were detectable in a few hours when isotope was administered shortly before visible differentiation of the gland. Measurements of nuclear size indicated no significant decreases in RNA-C(14) amount prior to the period of differentiation. During this later period, a large decrease in RNA-C(14) amount occurs suddenly, and the same amount of C(14) is added simultaneously to the DNA fraction, as expected if RNA-C(14) is utilized in the synthesis of DNA. The Rockefeller University Press 1959-05-01 /pmc/articles/PMC2224675/ /pubmed/13664687 Text en Copyright © Copyright, 1959, by The Rockefeller Institute |
spellingShingle | Article McMaster-Kaye, Rachel Taylor, J. Herbert The Metabolism of Chromosomal Ribonucleic Acid in Drosophila Salivary Glands and its Relation to Synthesis of Desoxyribonucleic Acid |
title | The Metabolism of Chromosomal Ribonucleic Acid in Drosophila Salivary Glands and its Relation to Synthesis of Desoxyribonucleic Acid |
title_full | The Metabolism of Chromosomal Ribonucleic Acid in Drosophila Salivary Glands and its Relation to Synthesis of Desoxyribonucleic Acid |
title_fullStr | The Metabolism of Chromosomal Ribonucleic Acid in Drosophila Salivary Glands and its Relation to Synthesis of Desoxyribonucleic Acid |
title_full_unstemmed | The Metabolism of Chromosomal Ribonucleic Acid in Drosophila Salivary Glands and its Relation to Synthesis of Desoxyribonucleic Acid |
title_short | The Metabolism of Chromosomal Ribonucleic Acid in Drosophila Salivary Glands and its Relation to Synthesis of Desoxyribonucleic Acid |
title_sort | metabolism of chromosomal ribonucleic acid in drosophila salivary glands and its relation to synthesis of desoxyribonucleic acid |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224675/ https://www.ncbi.nlm.nih.gov/pubmed/13664687 |
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