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Electron Microscopic Observations of Rabbit Antibodies
Electron micrographs were obtained showing the individual, shadow-cast macromolecules from solutions of purified anti-p-azobenzoate rabbit antibody and of normal γ-globulin. The two materials look alike and consist mainly of asymmetrical rod-like particles about 30 to 40 A in diameter. Lengths are n...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1959
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224689/ https://www.ncbi.nlm.nih.gov/pubmed/19866560 |
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author | Hall, C. E. Nisonoff, A. Slayter, H. S. |
author_facet | Hall, C. E. Nisonoff, A. Slayter, H. S. |
author_sort | Hall, C. E. |
collection | PubMed |
description | Electron micrographs were obtained showing the individual, shadow-cast macromolecules from solutions of purified anti-p-azobenzoate rabbit antibody and of normal γ-globulin. The two materials look alike and consist mainly of asymmetrical rod-like particles about 30 to 40 A in diameter. Lengths are not constant but the weight average is about 250 A for the antibodies and about 200 A for the γ-globulin. The average observed dimensions are reasonably consistent with values deduced from physical-chemical methods, although the shape is more nearly that of a cylindrical rod rather than the ellipsoid employed in hydrodynamical theory. Mixtures of antibody and specific dihaptenic dye were examined in attempts to establish the mode of the specific aggregation. At the high dilutions necessary for electron microscopy (0.1 mg./ml.), the effect of the dye was small and tended to be masked by non-specific aggregation on drying. The evidence suggests that under these conditions the specific reaction involves an end-to-end aggregation of the elementary particles to produce a weight average length about twice that of the pure antibody. |
format | Text |
id | pubmed-2224689 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1959 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22246892008-05-01 Electron Microscopic Observations of Rabbit Antibodies Hall, C. E. Nisonoff, A. Slayter, H. S. J Biophys Biochem Cytol Article Electron micrographs were obtained showing the individual, shadow-cast macromolecules from solutions of purified anti-p-azobenzoate rabbit antibody and of normal γ-globulin. The two materials look alike and consist mainly of asymmetrical rod-like particles about 30 to 40 A in diameter. Lengths are not constant but the weight average is about 250 A for the antibodies and about 200 A for the γ-globulin. The average observed dimensions are reasonably consistent with values deduced from physical-chemical methods, although the shape is more nearly that of a cylindrical rod rather than the ellipsoid employed in hydrodynamical theory. Mixtures of antibody and specific dihaptenic dye were examined in attempts to establish the mode of the specific aggregation. At the high dilutions necessary for electron microscopy (0.1 mg./ml.), the effect of the dye was small and tended to be masked by non-specific aggregation on drying. The evidence suggests that under these conditions the specific reaction involves an end-to-end aggregation of the elementary particles to produce a weight average length about twice that of the pure antibody. The Rockefeller University Press 1959-12-01 /pmc/articles/PMC2224689/ /pubmed/19866560 Text en Copyright © Copyright, 1959, by The Rockefeller Institute |
spellingShingle | Article Hall, C. E. Nisonoff, A. Slayter, H. S. Electron Microscopic Observations of Rabbit Antibodies |
title | Electron Microscopic Observations of Rabbit Antibodies |
title_full | Electron Microscopic Observations of Rabbit Antibodies |
title_fullStr | Electron Microscopic Observations of Rabbit Antibodies |
title_full_unstemmed | Electron Microscopic Observations of Rabbit Antibodies |
title_short | Electron Microscopic Observations of Rabbit Antibodies |
title_sort | electron microscopic observations of rabbit antibodies |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224689/ https://www.ncbi.nlm.nih.gov/pubmed/19866560 |
work_keys_str_mv | AT hallce electronmicroscopicobservationsofrabbitantibodies AT nisonoffa electronmicroscopicobservationsofrabbitantibodies AT slayterhs electronmicroscopicobservationsofrabbitantibodies |