A Diazo Coupling Method for the Electron Microscopic Localization of Cholinesterase

The presence of cholinesterase at the myoneural junction of intercostal muscle has been demonstrated in both light and electron microscopic preparations. A new simultaneous diazo coupling technique using α-naphthyl acetate as substrate and "hexazonium pararosanilin" as coupler has been app...

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Detalles Bibliográficos
Autores principales: Lehrer, Gerard M., Ornstein, Leonard
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1959
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224705/
https://www.ncbi.nlm.nih.gov/pubmed/14415404
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author Lehrer, Gerard M.
Ornstein, Leonard
author_facet Lehrer, Gerard M.
Ornstein, Leonard
author_sort Lehrer, Gerard M.
collection PubMed
description The presence of cholinesterase at the myoneural junction of intercostal muscle has been demonstrated in both light and electron microscopic preparations. A new simultaneous diazo coupling technique using α-naphthyl acetate as substrate and "hexazonium pararosanilin" as coupler has been applied to cold formalin-fixed tissues. After postfixation in buffered osmium tetroxide the sites of esterase activity are faithfully demonstrated at a high level of resolution. The details of cholin-esterase distribution and some technical aspects of the procedure are discussed.
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spelling pubmed-22247052008-05-01 A Diazo Coupling Method for the Electron Microscopic Localization of Cholinesterase Lehrer, Gerard M. Ornstein, Leonard J Biophys Biochem Cytol Article The presence of cholinesterase at the myoneural junction of intercostal muscle has been demonstrated in both light and electron microscopic preparations. A new simultaneous diazo coupling technique using α-naphthyl acetate as substrate and "hexazonium pararosanilin" as coupler has been applied to cold formalin-fixed tissues. After postfixation in buffered osmium tetroxide the sites of esterase activity are faithfully demonstrated at a high level of resolution. The details of cholin-esterase distribution and some technical aspects of the procedure are discussed. The Rockefeller University Press 1959-12-01 /pmc/articles/PMC2224705/ /pubmed/14415404 Text en Copyright © Copyright, 1959, by The Rockefeller Institute
spellingShingle Article
Lehrer, Gerard M.
Ornstein, Leonard
A Diazo Coupling Method for the Electron Microscopic Localization of Cholinesterase
title A Diazo Coupling Method for the Electron Microscopic Localization of Cholinesterase
title_full A Diazo Coupling Method for the Electron Microscopic Localization of Cholinesterase
title_fullStr A Diazo Coupling Method for the Electron Microscopic Localization of Cholinesterase
title_full_unstemmed A Diazo Coupling Method for the Electron Microscopic Localization of Cholinesterase
title_short A Diazo Coupling Method for the Electron Microscopic Localization of Cholinesterase
title_sort diazo coupling method for the electron microscopic localization of cholinesterase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224705/
https://www.ncbi.nlm.nih.gov/pubmed/14415404
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