EXTRACTION, HYDROLYSIS, AND ELECTROPHORETIC ANALYSIS OF RIBONUCLEIC ACID FROM MICROSCOPIC TISSUE UNITS (MICROPHORESIS)

A procedure for the purine-pyrimidine analysis of RNA in the 100- to 1000 µµg. range is presented. It includes hydrolysis and electrophoretic analysis of RNA, which is extracted from single isolated tissue units, like single cells. The quantitative determination of the separated compounds is carried...

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Autor principal: Edström, Jan-Erik
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1960
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224909/
https://www.ncbi.nlm.nih.gov/pubmed/13725765
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author Edström, Jan-Erik
author_facet Edström, Jan-Erik
author_sort Edström, Jan-Erik
collection PubMed
description A procedure for the purine-pyrimidine analysis of RNA in the 100- to 1000 µµg. range is presented. It includes hydrolysis and electrophoretic analysis of RNA, which is extracted from single isolated tissue units, like single cells. The quantitative determination of the separated compounds is carried out by a photographic-photometric procedure in ultraviolet light. The determined values show a coefficient of variation of about ±7 per cent on test substance. Microelectrophoretic analyses of RNA from different sources have been performed and are compared to macrochemical analyses. The agreement is good in those cases in which it is possible to get any information at all through macrochemical analyses.
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spelling pubmed-22249092008-05-01 EXTRACTION, HYDROLYSIS, AND ELECTROPHORETIC ANALYSIS OF RIBONUCLEIC ACID FROM MICROSCOPIC TISSUE UNITS (MICROPHORESIS) Edström, Jan-Erik J Biophys Biochem Cytol Article A procedure for the purine-pyrimidine analysis of RNA in the 100- to 1000 µµg. range is presented. It includes hydrolysis and electrophoretic analysis of RNA, which is extracted from single isolated tissue units, like single cells. The quantitative determination of the separated compounds is carried out by a photographic-photometric procedure in ultraviolet light. The determined values show a coefficient of variation of about ±7 per cent on test substance. Microelectrophoretic analyses of RNA from different sources have been performed and are compared to macrochemical analyses. The agreement is good in those cases in which it is possible to get any information at all through macrochemical analyses. The Rockefeller University Press 1960-09-01 /pmc/articles/PMC2224909/ /pubmed/13725765 Text en Copyright © Copyright 1961 by The Rockefeller Institute Press
spellingShingle Article
Edström, Jan-Erik
EXTRACTION, HYDROLYSIS, AND ELECTROPHORETIC ANALYSIS OF RIBONUCLEIC ACID FROM MICROSCOPIC TISSUE UNITS (MICROPHORESIS)
title EXTRACTION, HYDROLYSIS, AND ELECTROPHORETIC ANALYSIS OF RIBONUCLEIC ACID FROM MICROSCOPIC TISSUE UNITS (MICROPHORESIS)
title_full EXTRACTION, HYDROLYSIS, AND ELECTROPHORETIC ANALYSIS OF RIBONUCLEIC ACID FROM MICROSCOPIC TISSUE UNITS (MICROPHORESIS)
title_fullStr EXTRACTION, HYDROLYSIS, AND ELECTROPHORETIC ANALYSIS OF RIBONUCLEIC ACID FROM MICROSCOPIC TISSUE UNITS (MICROPHORESIS)
title_full_unstemmed EXTRACTION, HYDROLYSIS, AND ELECTROPHORETIC ANALYSIS OF RIBONUCLEIC ACID FROM MICROSCOPIC TISSUE UNITS (MICROPHORESIS)
title_short EXTRACTION, HYDROLYSIS, AND ELECTROPHORETIC ANALYSIS OF RIBONUCLEIC ACID FROM MICROSCOPIC TISSUE UNITS (MICROPHORESIS)
title_sort extraction, hydrolysis, and electrophoretic analysis of ribonucleic acid from microscopic tissue units (microphoresis)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224909/
https://www.ncbi.nlm.nih.gov/pubmed/13725765
work_keys_str_mv AT edstromjanerik extractionhydrolysisandelectrophoreticanalysisofribonucleicacidfrommicroscopictissueunitsmicrophoresis

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