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THE ISOLATION OF A CELL MEMBRANE FRACTION FROM RAT LIVER
A procedure is described for isolating cell membranes from rat liver homogenates. 20 gm. of rat liver was homogenized in a Dounce homogenizer in ice cold water buffered to pH 7.5 with NaHCO(3), rupturing all of the cells and most nuclei. The diluted homogenate was filtered through cheesecloth to rem...
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| Formato: | Texto |
| Lenguaje: | English |
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The Rockefeller University Press
1960
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224936/ https://www.ncbi.nlm.nih.gov/pubmed/13728607 |