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STUDIES ON SULFHYDRYL GROUPS DURING CELL DIVISION OF SEA URCHIN EGG : II. Mass Isolation of the Egg Cortex and Change in Its—SH Groups during Cell Division
Masses of cortices of both unfertilized and fertilized sea urchin eggs can be isolated by crushing eggs in hypotonic MaCl(2) (0.1 M) solution. The amount of cortical material in terms of protein-N increases steadily after fertilization until the monaster stage and thereafter remains almost constant...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1960
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224956/ https://www.ncbi.nlm.nih.gov/pubmed/13745476 |
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author | Sakai, Hikoichi |
author_facet | Sakai, Hikoichi |
author_sort | Sakai, Hikoichi |
collection | PubMed |
description | Masses of cortices of both unfertilized and fertilized sea urchin eggs can be isolated by crushing eggs in hypotonic MaCl(2) (0.1 M) solution. The amount of cortical material in terms of protein-N increases steadily after fertilization until the monaster stage and thereafter remains almost constant until well into the two-cell stage. The amount of bound—SH per protein-N of the egg cortex also increases after fertilization, reaches a maximum value at the amphiaster stage and thereafter decreases rapidly as the cleavage of the cell proceeds. |
format | Text |
id | pubmed-2224956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1960 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22249562008-05-01 STUDIES ON SULFHYDRYL GROUPS DURING CELL DIVISION OF SEA URCHIN EGG : II. Mass Isolation of the Egg Cortex and Change in Its—SH Groups during Cell Division Sakai, Hikoichi J Biophys Biochem Cytol Article Masses of cortices of both unfertilized and fertilized sea urchin eggs can be isolated by crushing eggs in hypotonic MaCl(2) (0.1 M) solution. The amount of cortical material in terms of protein-N increases steadily after fertilization until the monaster stage and thereafter remains almost constant until well into the two-cell stage. The amount of bound—SH per protein-N of the egg cortex also increases after fertilization, reaches a maximum value at the amphiaster stage and thereafter decreases rapidly as the cleavage of the cell proceeds. The Rockefeller University Press 1960-12-01 /pmc/articles/PMC2224956/ /pubmed/13745476 Text en Copyright © Copyright 1961 by The Rockefeller Institute Press |
spellingShingle | Article Sakai, Hikoichi STUDIES ON SULFHYDRYL GROUPS DURING CELL DIVISION OF SEA URCHIN EGG : II. Mass Isolation of the Egg Cortex and Change in Its—SH Groups during Cell Division |
title | STUDIES ON SULFHYDRYL GROUPS DURING CELL DIVISION OF SEA URCHIN EGG : II. Mass Isolation of the Egg Cortex and Change in Its—SH Groups during Cell Division |
title_full | STUDIES ON SULFHYDRYL GROUPS DURING CELL DIVISION OF SEA URCHIN EGG : II. Mass Isolation of the Egg Cortex and Change in Its—SH Groups during Cell Division |
title_fullStr | STUDIES ON SULFHYDRYL GROUPS DURING CELL DIVISION OF SEA URCHIN EGG : II. Mass Isolation of the Egg Cortex and Change in Its—SH Groups during Cell Division |
title_full_unstemmed | STUDIES ON SULFHYDRYL GROUPS DURING CELL DIVISION OF SEA URCHIN EGG : II. Mass Isolation of the Egg Cortex and Change in Its—SH Groups during Cell Division |
title_short | STUDIES ON SULFHYDRYL GROUPS DURING CELL DIVISION OF SEA URCHIN EGG : II. Mass Isolation of the Egg Cortex and Change in Its—SH Groups during Cell Division |
title_sort | studies on sulfhydryl groups during cell division of sea urchin egg : ii. mass isolation of the egg cortex and change in its—sh groups during cell division |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224956/ https://www.ncbi.nlm.nih.gov/pubmed/13745476 |
work_keys_str_mv | AT sakaihikoichi studiesonsulfhydrylgroupsduringcelldivisionofseaurchineggiimassisolationoftheeggcortexandchangeinitsshgroupsduringcelldivision |