IRRADIATION ULTRA-VIOLETTE DES ORGANITES CELLULAIRES AVEC OBSERVATION CONTINUE EN CONTRASTE DE PHASE

The apparatus described is designed to permit the observation of the smallest cellular components with the phase contrast microscope (which requires an objective of a high numerical aperture) while a part of the cell is irradiated by a very small spot of ultraviolet radiation. Theoretical considerat...

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Detalles Bibliográficos
Autores principales: Bessis, Marcel, Nomarski, Georges
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1960
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224964/
https://www.ncbi.nlm.nih.gov/pubmed/19866576
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author Bessis, Marcel
Nomarski, Georges
author_facet Bessis, Marcel
Nomarski, Georges
author_sort Bessis, Marcel
collection PubMed
description The apparatus described is designed to permit the observation of the smallest cellular components with the phase contrast microscope (which requires an objective of a high numerical aperture) while a part of the cell is irradiated by a very small spot of ultraviolet radiation. Theoretical considerations show a good distribution of the energy for a diameter of the irradiation spot as small as 0.2 microns. Indications are given about the limitations of the brightness caused by diffraction at the spot diaphragm and the energy absorption by volume-unit of the irradiated object. The practical applications of the apparatus are discussed.
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spelling pubmed-22249642008-05-01 IRRADIATION ULTRA-VIOLETTE DES ORGANITES CELLULAIRES AVEC OBSERVATION CONTINUE EN CONTRASTE DE PHASE Bessis, Marcel Nomarski, Georges J Biophys Biochem Cytol Article The apparatus described is designed to permit the observation of the smallest cellular components with the phase contrast microscope (which requires an objective of a high numerical aperture) while a part of the cell is irradiated by a very small spot of ultraviolet radiation. Theoretical considerations show a good distribution of the energy for a diameter of the irradiation spot as small as 0.2 microns. Indications are given about the limitations of the brightness caused by diffraction at the spot diaphragm and the energy absorption by volume-unit of the irradiated object. The practical applications of the apparatus are discussed. The Rockefeller University Press 1960-12-01 /pmc/articles/PMC2224964/ /pubmed/19866576 Text en Copyright © Copyright 1961 by The Rockefeller Institute Press
spellingShingle Article
Bessis, Marcel
Nomarski, Georges
IRRADIATION ULTRA-VIOLETTE DES ORGANITES CELLULAIRES AVEC OBSERVATION CONTINUE EN CONTRASTE DE PHASE
title IRRADIATION ULTRA-VIOLETTE DES ORGANITES CELLULAIRES AVEC OBSERVATION CONTINUE EN CONTRASTE DE PHASE
title_full IRRADIATION ULTRA-VIOLETTE DES ORGANITES CELLULAIRES AVEC OBSERVATION CONTINUE EN CONTRASTE DE PHASE
title_fullStr IRRADIATION ULTRA-VIOLETTE DES ORGANITES CELLULAIRES AVEC OBSERVATION CONTINUE EN CONTRASTE DE PHASE
title_full_unstemmed IRRADIATION ULTRA-VIOLETTE DES ORGANITES CELLULAIRES AVEC OBSERVATION CONTINUE EN CONTRASTE DE PHASE
title_short IRRADIATION ULTRA-VIOLETTE DES ORGANITES CELLULAIRES AVEC OBSERVATION CONTINUE EN CONTRASTE DE PHASE
title_sort irradiation ultra-violette des organites cellulaires avec observation continue en contraste de phase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224964/
https://www.ncbi.nlm.nih.gov/pubmed/19866576
work_keys_str_mv AT bessismarcel irradiationultraviolettedesorganitescellulairesavecobservationcontinueencontrastedephase
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