STUDIES ON THE PROBLEM OF PRESERVATION OF MYELIN SHEATH ULTRASTRUCTURE: EVALUATION OF FIXATION, DEHYDRATION, AND EMBEDDING TECHNIQUES

Currently accepted methods of tissue preparation for electron microscopy result in alterations of myelinated nerve fibers. In an attempt to minimize distortion of myelin, various fixation techniques, dehydration schedules, and embedding methods have been evaluated. It was found that the major damage...

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Detalles Bibliográficos
Autores principales: Condie, Richard M., Howell, A. Ervin, Good, Robert A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1961
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225008/
https://www.ncbi.nlm.nih.gov/pubmed/19866582
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author Condie, Richard M.
Howell, A. Ervin
Good, Robert A.
author_facet Condie, Richard M.
Howell, A. Ervin
Good, Robert A.
author_sort Condie, Richard M.
collection PubMed
description Currently accepted methods of tissue preparation for electron microscopy result in alterations of myelinated nerve fibers. In an attempt to minimize distortion of myelin, various fixation techniques, dehydration schedules, and embedding methods have been evaluated. It was found that the major damage to myelinated nerves occurs in the embedding procedure. A technique for embedding nerve tissue using the polyester Vestopal W is described which was found to result in improved preservation of myelin.
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spelling pubmed-22250082008-05-01 STUDIES ON THE PROBLEM OF PRESERVATION OF MYELIN SHEATH ULTRASTRUCTURE: EVALUATION OF FIXATION, DEHYDRATION, AND EMBEDDING TECHNIQUES Condie, Richard M. Howell, A. Ervin Good, Robert A. J Biophys Biochem Cytol Article Currently accepted methods of tissue preparation for electron microscopy result in alterations of myelinated nerve fibers. In an attempt to minimize distortion of myelin, various fixation techniques, dehydration schedules, and embedding methods have been evaluated. It was found that the major damage to myelinated nerves occurs in the embedding procedure. A technique for embedding nerve tissue using the polyester Vestopal W is described which was found to result in improved preservation of myelin. The Rockefeller University Press 1961-02-01 /pmc/articles/PMC2225008/ /pubmed/19866582 Text en Copyright © Copyright, 1961, by The Rockefeller Institute Press
spellingShingle Article
Condie, Richard M.
Howell, A. Ervin
Good, Robert A.
STUDIES ON THE PROBLEM OF PRESERVATION OF MYELIN SHEATH ULTRASTRUCTURE: EVALUATION OF FIXATION, DEHYDRATION, AND EMBEDDING TECHNIQUES
title STUDIES ON THE PROBLEM OF PRESERVATION OF MYELIN SHEATH ULTRASTRUCTURE: EVALUATION OF FIXATION, DEHYDRATION, AND EMBEDDING TECHNIQUES
title_full STUDIES ON THE PROBLEM OF PRESERVATION OF MYELIN SHEATH ULTRASTRUCTURE: EVALUATION OF FIXATION, DEHYDRATION, AND EMBEDDING TECHNIQUES
title_fullStr STUDIES ON THE PROBLEM OF PRESERVATION OF MYELIN SHEATH ULTRASTRUCTURE: EVALUATION OF FIXATION, DEHYDRATION, AND EMBEDDING TECHNIQUES
title_full_unstemmed STUDIES ON THE PROBLEM OF PRESERVATION OF MYELIN SHEATH ULTRASTRUCTURE: EVALUATION OF FIXATION, DEHYDRATION, AND EMBEDDING TECHNIQUES
title_short STUDIES ON THE PROBLEM OF PRESERVATION OF MYELIN SHEATH ULTRASTRUCTURE: EVALUATION OF FIXATION, DEHYDRATION, AND EMBEDDING TECHNIQUES
title_sort studies on the problem of preservation of myelin sheath ultrastructure: evaluation of fixation, dehydration, and embedding techniques
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225008/
https://www.ncbi.nlm.nih.gov/pubmed/19866582
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