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STUDIES ON THE OXIDATIVE METABOLISM OF SACCHAROMYCES CEREVISIAE : II. Morphology and Oxidative Phosphorylation Capacity of Mitochondria and Derived Particles from Baker's Yeast

A specially designed high-speed blendor and glass beads have been used to disintegrate yeast cells. The method enables large quantities of cells to be fragmented quickly at low temperature, and cell-free mitochondrial particles to be prepared in high yield. The particles are isolated in a sucrose-Tr...

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Detalles Bibliográficos
Autores principales: Vitols, Eberhards, Linnane, Anthony W.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1961
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225020/
https://www.ncbi.nlm.nih.gov/pubmed/13781661
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author Vitols, Eberhards
Linnane, Anthony W.
author_facet Vitols, Eberhards
Linnane, Anthony W.
author_sort Vitols, Eberhards
collection PubMed
description A specially designed high-speed blendor and glass beads have been used to disintegrate yeast cells. The method enables large quantities of cells to be fragmented quickly at low temperature, and cell-free mitochondrial particles to be prepared in high yield. The particles are isolated in a sucrose-Tris-EDTA medium and extensively refractionated in the same medium. The success of the fractionation is dependent upon the presence of the Tris buffer, as the latter prevents the aggregation of the particulate material. Two morphologically and enzymatically different particle types have been obtained: a heavy fraction corresponding to mitochondria in size and internal organization, and a light fraction consisting of vesicular, single-membrane particles of a smaller size. The light particles oxidize DPNH and succinate, but do not oxidize pyruvate-malate, and lack the capacity for phosphorylation. The heavy particles oxidize pyruvate-malate as well as the citric acid cycle intermediates, although their α-ketoglutaric dehydrogenase activity is low. Oxidation by the heavy particles is coupled to phosphorylation, and P/O ratios of about 1.5 have been obtained. Lactic acid dehydrogenase is also present in the heavy fraction, and lactate is oxidized with a P/O ratio of about 0.7.
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spelling pubmed-22250202008-05-01 STUDIES ON THE OXIDATIVE METABOLISM OF SACCHAROMYCES CEREVISIAE : II. Morphology and Oxidative Phosphorylation Capacity of Mitochondria and Derived Particles from Baker's Yeast Vitols, Eberhards Linnane, Anthony W. J Biophys Biochem Cytol Article A specially designed high-speed blendor and glass beads have been used to disintegrate yeast cells. The method enables large quantities of cells to be fragmented quickly at low temperature, and cell-free mitochondrial particles to be prepared in high yield. The particles are isolated in a sucrose-Tris-EDTA medium and extensively refractionated in the same medium. The success of the fractionation is dependent upon the presence of the Tris buffer, as the latter prevents the aggregation of the particulate material. Two morphologically and enzymatically different particle types have been obtained: a heavy fraction corresponding to mitochondria in size and internal organization, and a light fraction consisting of vesicular, single-membrane particles of a smaller size. The light particles oxidize DPNH and succinate, but do not oxidize pyruvate-malate, and lack the capacity for phosphorylation. The heavy particles oxidize pyruvate-malate as well as the citric acid cycle intermediates, although their α-ketoglutaric dehydrogenase activity is low. Oxidation by the heavy particles is coupled to phosphorylation, and P/O ratios of about 1.5 have been obtained. Lactic acid dehydrogenase is also present in the heavy fraction, and lactate is oxidized with a P/O ratio of about 0.7. The Rockefeller University Press 1961-03-01 /pmc/articles/PMC2225020/ /pubmed/13781661 Text en Copyright © Copyright, 1961, by The Rockefeller Institute Press
spellingShingle Article
Vitols, Eberhards
Linnane, Anthony W.
STUDIES ON THE OXIDATIVE METABOLISM OF SACCHAROMYCES CEREVISIAE : II. Morphology and Oxidative Phosphorylation Capacity of Mitochondria and Derived Particles from Baker's Yeast
title STUDIES ON THE OXIDATIVE METABOLISM OF SACCHAROMYCES CEREVISIAE : II. Morphology and Oxidative Phosphorylation Capacity of Mitochondria and Derived Particles from Baker's Yeast
title_full STUDIES ON THE OXIDATIVE METABOLISM OF SACCHAROMYCES CEREVISIAE : II. Morphology and Oxidative Phosphorylation Capacity of Mitochondria and Derived Particles from Baker's Yeast
title_fullStr STUDIES ON THE OXIDATIVE METABOLISM OF SACCHAROMYCES CEREVISIAE : II. Morphology and Oxidative Phosphorylation Capacity of Mitochondria and Derived Particles from Baker's Yeast
title_full_unstemmed STUDIES ON THE OXIDATIVE METABOLISM OF SACCHAROMYCES CEREVISIAE : II. Morphology and Oxidative Phosphorylation Capacity of Mitochondria and Derived Particles from Baker's Yeast
title_short STUDIES ON THE OXIDATIVE METABOLISM OF SACCHAROMYCES CEREVISIAE : II. Morphology and Oxidative Phosphorylation Capacity of Mitochondria and Derived Particles from Baker's Yeast
title_sort studies on the oxidative metabolism of saccharomyces cerevisiae : ii. morphology and oxidative phosphorylation capacity of mitochondria and derived particles from baker's yeast
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225020/
https://www.ncbi.nlm.nih.gov/pubmed/13781661
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