A NEW FREEZING-ULTRAMICROTOME

The difficulties in sectioning frozen biological objects for electron microscopic investigations are overcome by Steere's freezing-etching method. In order to test this method and to open up a wide field of application, the new freezing-ultramicrotome has been designed. The apparatus consists o...

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Detalles Bibliográficos
Autores principales: Moor, H., Mühlethaler, K., Waldner, H., Frey-Wyssling, A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1961
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225066/
https://www.ncbi.nlm.nih.gov/pubmed/13772269
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author Moor, H.
Mühlethaler, K.
Waldner, H.
Frey-Wyssling, A.
author_facet Moor, H.
Mühlethaler, K.
Waldner, H.
Frey-Wyssling, A.
author_sort Moor, H.
collection PubMed
description The difficulties in sectioning frozen biological objects for electron microscopic investigations are overcome by Steere's freezing-etching method. In order to test this method and to open up a wide field of application, the new freezing-ultramicrotome has been designed. The apparatus consists of the combination of an ultramicrotome with freezing-drying and shadow-casting installations in the same vacuum container. The preliminary results show, on the one hand, the practicability of all preparational steps and, on the other, that it is possible to resolve internal structures of cell organelles and even macromolecular patterns.
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spelling pubmed-22250662008-05-01 A NEW FREEZING-ULTRAMICROTOME Moor, H. Mühlethaler, K. Waldner, H. Frey-Wyssling, A. J Biophys Biochem Cytol Article The difficulties in sectioning frozen biological objects for electron microscopic investigations are overcome by Steere's freezing-etching method. In order to test this method and to open up a wide field of application, the new freezing-ultramicrotome has been designed. The apparatus consists of the combination of an ultramicrotome with freezing-drying and shadow-casting installations in the same vacuum container. The preliminary results show, on the one hand, the practicability of all preparational steps and, on the other, that it is possible to resolve internal structures of cell organelles and even macromolecular patterns. The Rockefeller University Press 1961-05-01 /pmc/articles/PMC2225066/ /pubmed/13772269 Text en Copyright © Copyright, 1961, by The Rockefeller Institute Press
spellingShingle Article
Moor, H.
Mühlethaler, K.
Waldner, H.
Frey-Wyssling, A.
A NEW FREEZING-ULTRAMICROTOME
title A NEW FREEZING-ULTRAMICROTOME
title_full A NEW FREEZING-ULTRAMICROTOME
title_fullStr A NEW FREEZING-ULTRAMICROTOME
title_full_unstemmed A NEW FREEZING-ULTRAMICROTOME
title_short A NEW FREEZING-ULTRAMICROTOME
title_sort new freezing-ultramicrotome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225066/
https://www.ncbi.nlm.nih.gov/pubmed/13772269
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