SOME THEORETICAL ASPECTS OF OSMIUM TETROXIDE FIXATION WITH SPECIAL REFERENCE TO THE METAPHASE CHROMOSOMES OF CELL CULTURES

Fixation of cell cultures with 1 per cent OsO(4) at constant pH and tonicity but variable cationic valence and dielectric constant causes profound changes in metaphase chromosomes. It is possible to make them disappear, flocculate, or show little change from the living cell in the phase contrast mic...

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Detalles Bibliográficos
Autor principal: Robbins, Elliott
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1961
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225163/
https://www.ncbi.nlm.nih.gov/pubmed/14492680
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author Robbins, Elliott
author_facet Robbins, Elliott
author_sort Robbins, Elliott
collection PubMed
description Fixation of cell cultures with 1 per cent OsO(4) at constant pH and tonicity but variable cationic valence and dielectric constant causes profound changes in metaphase chromosomes. It is possible to make them disappear, flocculate, or show little change from the living cell in the phase contrast microscope. Conventional fixation for the electron microscope causes almost complete disappearance of metaphase chromosomes in phase contrast. Reasons for this behavior are discussed. It is postulated that intermolecular distances and consequently internal structure in chromosomes are governed by the same forces that govern these distances in colloidal sols.
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spelling pubmed-22251632008-05-01 SOME THEORETICAL ASPECTS OF OSMIUM TETROXIDE FIXATION WITH SPECIAL REFERENCE TO THE METAPHASE CHROMOSOMES OF CELL CULTURES Robbins, Elliott J Biophys Biochem Cytol Article Fixation of cell cultures with 1 per cent OsO(4) at constant pH and tonicity but variable cationic valence and dielectric constant causes profound changes in metaphase chromosomes. It is possible to make them disappear, flocculate, or show little change from the living cell in the phase contrast microscope. Conventional fixation for the electron microscope causes almost complete disappearance of metaphase chromosomes in phase contrast. Reasons for this behavior are discussed. It is postulated that intermolecular distances and consequently internal structure in chromosomes are governed by the same forces that govern these distances in colloidal sols. The Rockefeller University Press 1961-11-01 /pmc/articles/PMC2225163/ /pubmed/14492680 Text en Copyright © Copyright, 1961, by The Rockefeller Institute Press
spellingShingle Article
Robbins, Elliott
SOME THEORETICAL ASPECTS OF OSMIUM TETROXIDE FIXATION WITH SPECIAL REFERENCE TO THE METAPHASE CHROMOSOMES OF CELL CULTURES
title SOME THEORETICAL ASPECTS OF OSMIUM TETROXIDE FIXATION WITH SPECIAL REFERENCE TO THE METAPHASE CHROMOSOMES OF CELL CULTURES
title_full SOME THEORETICAL ASPECTS OF OSMIUM TETROXIDE FIXATION WITH SPECIAL REFERENCE TO THE METAPHASE CHROMOSOMES OF CELL CULTURES
title_fullStr SOME THEORETICAL ASPECTS OF OSMIUM TETROXIDE FIXATION WITH SPECIAL REFERENCE TO THE METAPHASE CHROMOSOMES OF CELL CULTURES
title_full_unstemmed SOME THEORETICAL ASPECTS OF OSMIUM TETROXIDE FIXATION WITH SPECIAL REFERENCE TO THE METAPHASE CHROMOSOMES OF CELL CULTURES
title_short SOME THEORETICAL ASPECTS OF OSMIUM TETROXIDE FIXATION WITH SPECIAL REFERENCE TO THE METAPHASE CHROMOSOMES OF CELL CULTURES
title_sort some theoretical aspects of osmium tetroxide fixation with special reference to the metaphase chromosomes of cell cultures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225163/
https://www.ncbi.nlm.nih.gov/pubmed/14492680
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