Increased Mutation Rate of E. coli K12λ Cultures Maintained in Continuous Logarithmic Growth

Continuous logarithmic growth of E. coli K12λ in an automatic culture cell resulted in marked increases in the proportion of several mutants. The P(1) phage-resistant cells increased 10 to 3000 times, the T(2) phage-resistant cells 1 to 1000 times, the neomycin-resistant cells 1 to 10 times, and the...

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Detalles Bibliográficos
Autor principal: Northrop, John H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1966
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225649/
https://www.ncbi.nlm.nih.gov/pubmed/11526835
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author Northrop, John H.
author_facet Northrop, John H.
author_sort Northrop, John H.
collection PubMed
description Continuous logarithmic growth of E. coli K12λ in an automatic culture cell resulted in marked increases in the proportion of several mutants. The P(1) phage-resistant cells increased 10 to 3000 times, the T(2) phage-resistant cells 1 to 1000 times, the neomycin-resistant cells 1 to 10 times, and the virus-producing cells 30 to 70 times. No change occurred in the penicillin-resistant cells. Calculation of the growth curves and direct determination of the mutation rates by the null fraction method showed that the increases in the proportion of mutants were due to increases in the mutation rates.
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spelling pubmed-22256492008-04-23 Increased Mutation Rate of E. coli K12λ Cultures Maintained in Continuous Logarithmic Growth Northrop, John H. J Gen Physiol Article Continuous logarithmic growth of E. coli K12λ in an automatic culture cell resulted in marked increases in the proportion of several mutants. The P(1) phage-resistant cells increased 10 to 3000 times, the T(2) phage-resistant cells 1 to 1000 times, the neomycin-resistant cells 1 to 10 times, and the virus-producing cells 30 to 70 times. No change occurred in the penicillin-resistant cells. Calculation of the growth curves and direct determination of the mutation rates by the null fraction method showed that the increases in the proportion of mutants were due to increases in the mutation rates. The Rockefeller University Press 1966-11-01 /pmc/articles/PMC2225649/ /pubmed/11526835 Text en Copyright © 1967 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Northrop, John H.
Increased Mutation Rate of E. coli K12λ Cultures Maintained in Continuous Logarithmic Growth
title Increased Mutation Rate of E. coli K12λ Cultures Maintained in Continuous Logarithmic Growth
title_full Increased Mutation Rate of E. coli K12λ Cultures Maintained in Continuous Logarithmic Growth
title_fullStr Increased Mutation Rate of E. coli K12λ Cultures Maintained in Continuous Logarithmic Growth
title_full_unstemmed Increased Mutation Rate of E. coli K12λ Cultures Maintained in Continuous Logarithmic Growth
title_short Increased Mutation Rate of E. coli K12λ Cultures Maintained in Continuous Logarithmic Growth
title_sort increased mutation rate of e. coli k12λ cultures maintained in continuous logarithmic growth
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225649/
https://www.ncbi.nlm.nih.gov/pubmed/11526835
work_keys_str_mv AT northropjohnh increasedmutationrateofecolik12lculturesmaintainedincontinuouslogarithmicgrowth

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