Oxygen Consumption Rate of Tissue Measured by a Micropolarographic Method

A new method for measuring the oxygen consumption rate of a sheet of homogeneous tissue is described. The method measures, by a Clark-type oxygen electrode without a membrane, the time for the tissue to consume all its dissolved oxygen. The electrode is applied to one surface of the tissue sheet and...

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Detalles Bibliográficos
Autores principales: Takahashi, G. H., Fatt, I., Goldstick, T. K.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1966
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225657/
https://www.ncbi.nlm.nih.gov/pubmed/11526831
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author Takahashi, G. H.
Fatt, I.
Goldstick, T. K.
author_facet Takahashi, G. H.
Fatt, I.
Goldstick, T. K.
author_sort Takahashi, G. H.
collection PubMed
description A new method for measuring the oxygen consumption rate of a sheet of homogeneous tissue is described. The method measures, by a Clark-type oxygen electrode without a membrane, the time for the tissue to consume all its dissolved oxygen. The electrode is applied to one surface of the tissue sheet and the other surface is sealed from the atmosphere by a cover slip. The consumption is calculated from an estimate of the oxygen dissolved in the tissue at the moment it is covered and the time for the oxygen tension at one surface to fall to zero. The data also yield the oxygen diffusion coefficient in the oxygen-consuming tissue.
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spelling pubmed-22256572008-04-23 Oxygen Consumption Rate of Tissue Measured by a Micropolarographic Method Takahashi, G. H. Fatt, I. Goldstick, T. K. J Gen Physiol Article A new method for measuring the oxygen consumption rate of a sheet of homogeneous tissue is described. The method measures, by a Clark-type oxygen electrode without a membrane, the time for the tissue to consume all its dissolved oxygen. The electrode is applied to one surface of the tissue sheet and the other surface is sealed from the atmosphere by a cover slip. The consumption is calculated from an estimate of the oxygen dissolved in the tissue at the moment it is covered and the time for the oxygen tension at one surface to fall to zero. The data also yield the oxygen diffusion coefficient in the oxygen-consuming tissue. The Rockefeller University Press 1966-11-01 /pmc/articles/PMC2225657/ /pubmed/11526831 Text en Copyright © 1967 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Takahashi, G. H.
Fatt, I.
Goldstick, T. K.
Oxygen Consumption Rate of Tissue Measured by a Micropolarographic Method
title Oxygen Consumption Rate of Tissue Measured by a Micropolarographic Method
title_full Oxygen Consumption Rate of Tissue Measured by a Micropolarographic Method
title_fullStr Oxygen Consumption Rate of Tissue Measured by a Micropolarographic Method
title_full_unstemmed Oxygen Consumption Rate of Tissue Measured by a Micropolarographic Method
title_short Oxygen Consumption Rate of Tissue Measured by a Micropolarographic Method
title_sort oxygen consumption rate of tissue measured by a micropolarographic method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225657/
https://www.ncbi.nlm.nih.gov/pubmed/11526831
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AT fatti oxygenconsumptionrateoftissuemeasuredbyamicropolarographicmethod
AT goldsticktk oxygenconsumptionrateoftissuemeasuredbyamicropolarographicmethod

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