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Permeability Studies on Red Cell Membranes of Dog, Cat, and Beef

Water permeability coefficients of dog, cat, and beef red cell membranes have been measured under an osmotic pressure gradient. The measurements employed a rapid reaction stop flow apparatus with which cell shrinking was measured under a relative osmotic pressure gradient of 1.25 to 1.64 times the i...

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Detalles Bibliográficos
Autores principales: Rich, G. T., Sha'afi, R. I., Barton, T. C., Solomon, A. K.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1967
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225667/
https://www.ncbi.nlm.nih.gov/pubmed/6063688
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author Rich, G. T.
Sha'afi, R. I.
Barton, T. C.
Solomon, A. K.
author_facet Rich, G. T.
Sha'afi, R. I.
Barton, T. C.
Solomon, A. K.
author_sort Rich, G. T.
collection PubMed
description Water permeability coefficients of dog, cat, and beef red cell membranes have been measured under an osmotic pressure gradient. The measurements employed a rapid reaction stop flow apparatus with which cell shrinking was measured under a relative osmotic pressure gradient of 1.25 to 1.64 times the isosmolar concentration. For the dog red cell the osmotic permeability coefficient is 0.36 cm(4)/(sec, osmol). The water permeability coefficient for the dog red cell under a diffusion gradient was also measured (rate constant = 0.10/msec). The ratio between the two permeabilities was used to calculate an equivalent pore radius of 5.9 A. This value agrees welt with an equivalent pore radius of 6.2 A obtained from reflection coefficients of nonelectrolyte water-soluble molecules, and is consistent with data on the permeability of the dog red cell membrane to glucose. These data provide evidence supporting the existence of equivalent pores in single biological membranes.
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spelling pubmed-22256672008-04-23 Permeability Studies on Red Cell Membranes of Dog, Cat, and Beef Rich, G. T. Sha'afi, R. I. Barton, T. C. Solomon, A. K. J Gen Physiol Article Water permeability coefficients of dog, cat, and beef red cell membranes have been measured under an osmotic pressure gradient. The measurements employed a rapid reaction stop flow apparatus with which cell shrinking was measured under a relative osmotic pressure gradient of 1.25 to 1.64 times the isosmolar concentration. For the dog red cell the osmotic permeability coefficient is 0.36 cm(4)/(sec, osmol). The water permeability coefficient for the dog red cell under a diffusion gradient was also measured (rate constant = 0.10/msec). The ratio between the two permeabilities was used to calculate an equivalent pore radius of 5.9 A. This value agrees welt with an equivalent pore radius of 6.2 A obtained from reflection coefficients of nonelectrolyte water-soluble molecules, and is consistent with data on the permeability of the dog red cell membrane to glucose. These data provide evidence supporting the existence of equivalent pores in single biological membranes. The Rockefeller University Press 1967-11-01 /pmc/articles/PMC2225667/ /pubmed/6063688 Text en Copyright © 1967 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Rich, G. T.
Sha'afi, R. I.
Barton, T. C.
Solomon, A. K.
Permeability Studies on Red Cell Membranes of Dog, Cat, and Beef
title Permeability Studies on Red Cell Membranes of Dog, Cat, and Beef
title_full Permeability Studies on Red Cell Membranes of Dog, Cat, and Beef
title_fullStr Permeability Studies on Red Cell Membranes of Dog, Cat, and Beef
title_full_unstemmed Permeability Studies on Red Cell Membranes of Dog, Cat, and Beef
title_short Permeability Studies on Red Cell Membranes of Dog, Cat, and Beef
title_sort permeability studies on red cell membranes of dog, cat, and beef
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225667/
https://www.ncbi.nlm.nih.gov/pubmed/6063688
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