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Cellular Mechanisms of Interferon Production
Rabbit kidney cell cultures stimulated with either double-stranded polyinosinate-polycytidylate (poly I:poly C) or with ultraviolet-irradiated Newcastle disease virus (UV-NDV) produce two types of interferon response, designated "early" and "late," respectively. The early respons...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1970
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225870/ https://www.ncbi.nlm.nih.gov/pubmed/19873676 |
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author | Vilček, Jan |
author_facet | Vilček, Jan |
author_sort | Vilček, Jan |
collection | PubMed |
description | Rabbit kidney cell cultures stimulated with either double-stranded polyinosinate-polycytidylate (poly I:poly C) or with ultraviolet-irradiated Newcastle disease virus (UV-NDV) produce two types of interferon response, designated "early" and "late," respectively. The early response is suppressed by inhibitors of RNA or protein synthesis and is therefore thought to represent de novo synthesis of interferon. Circumstantial evidence suggested that this interferon response is regulated by a translation control mechanism. Late interferon production with poly I:poly C only took place in the presence of inhibitors of RNA or protein synthesis. The late interferon is therefore likely to be derived by the activation of an interferon precursor. The stimulation of late poly I:poly C-induced interferon production by cycloheximide suggested the existence of a second, posttranslational level of control of interferon production. This posttranslation control seems to be activated by interferon. UV-NDV can probably suppress the synthesis of the posttranslation inhibitory protein, and therefore it stimulates a late interferon response in the absence of inhibitors of RNA or protein synthesis. It is postulated that both the translation and posttranslation inhibitor participate in the development of a cellular refractory state to repeated interferon stimulation. The picture of interferon which emerges from this study is one of a heterogenous class of proteins whose production is controlled by cellular repressors acting at various levels. |
format | Text |
id | pubmed-2225870 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1970 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22258702008-04-23 Cellular Mechanisms of Interferon Production Vilček, Jan J Gen Physiol Interferon Induction ⋅ II Rabbit kidney cell cultures stimulated with either double-stranded polyinosinate-polycytidylate (poly I:poly C) or with ultraviolet-irradiated Newcastle disease virus (UV-NDV) produce two types of interferon response, designated "early" and "late," respectively. The early response is suppressed by inhibitors of RNA or protein synthesis and is therefore thought to represent de novo synthesis of interferon. Circumstantial evidence suggested that this interferon response is regulated by a translation control mechanism. Late interferon production with poly I:poly C only took place in the presence of inhibitors of RNA or protein synthesis. The late interferon is therefore likely to be derived by the activation of an interferon precursor. The stimulation of late poly I:poly C-induced interferon production by cycloheximide suggested the existence of a second, posttranslational level of control of interferon production. This posttranslation control seems to be activated by interferon. UV-NDV can probably suppress the synthesis of the posttranslation inhibitory protein, and therefore it stimulates a late interferon response in the absence of inhibitors of RNA or protein synthesis. It is postulated that both the translation and posttranslation inhibitor participate in the development of a cellular refractory state to repeated interferon stimulation. The picture of interferon which emerges from this study is one of a heterogenous class of proteins whose production is controlled by cellular repressors acting at various levels. The Rockefeller University Press 1970-07-01 /pmc/articles/PMC2225870/ /pubmed/19873676 Text en Copyright © 1970 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Interferon Induction ⋅ II Vilček, Jan Cellular Mechanisms of Interferon Production |
title | Cellular Mechanisms of Interferon Production |
title_full | Cellular Mechanisms of Interferon Production |
title_fullStr | Cellular Mechanisms of Interferon Production |
title_full_unstemmed | Cellular Mechanisms of Interferon Production |
title_short | Cellular Mechanisms of Interferon Production |
title_sort | cellular mechanisms of interferon production |
topic | Interferon Induction ⋅ II |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225870/ https://www.ncbi.nlm.nih.gov/pubmed/19873676 |
work_keys_str_mv | AT vilcekjan cellularmechanismsofinterferonproduction |