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Ion Transport and the Development of Hydrogen Ion Secretion in the Stomach of the Metamorphosing Bullfrog Tadpole
Isolated bullfrog tadpole stomachs secrete H(+) by stage XXIV of metamorphosis, when tail reabsorption is nearly complete. At this stage the PD shows characteristic responses identical to those of the adult. The appearance of HCl secretion correlates well with other studies showing the morphogenesis...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1969
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225896/ https://www.ncbi.nlm.nih.gov/pubmed/5792366 |
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author | Forte, John G. Limlomwongse, Liangchai Kasbekar, Dinkar K. |
author_facet | Forte, John G. Limlomwongse, Liangchai Kasbekar, Dinkar K. |
author_sort | Forte, John G. |
collection | PubMed |
description | Isolated bullfrog tadpole stomachs secrete H(+) by stage XXIV of metamorphosis, when tail reabsorption is nearly complete. At this stage the PD shows characteristic responses identical to those of the adult. The appearance of HCl secretion correlates well with other studies showing the morphogenesis of oxyntic cells. Prior to the development of H(+) secretion tadpole stomachs maintain a PD similar in polarity and magnitude to that of the adult; i.e., secretory (S) side negative with respect to the nutrient (N) side. The interdependence with aerobic metabolism appeared to increase progressively through metamorphosis; however, glycolytic inhibitors always abolished the PD. Isotopic flux analysis showed that the transepithelial movement of Na(+) was consistent with passive diffusion, whereas an active transport of Cl(-) from N to S was clearly indicated. Variations in [Na(+)], [K(+)], and [Cl(-)] in the bathing solutions induced changes consistent with the following functional description of the pre-H(+)-secreting tadpole stomach. (a) The S side is relatively permeable to Cl(-), but not to Na(+) or K(+). (b) An equilibrium potential for K(+) and Cl(-) exists at the N interface. (c) Ouabain abolishes the selective K(+) permeablity at the N interface and reduces the total PD. (d) Effects of Na(+) replacement by choline in the N solution become manifest only below 10–20 mM. It is concluded that prior to development of H(+) secretion, the tadpole gastric PD is generated by a Cl(-) pump from N to S and a Na(+) pump operating from the cell interior toward the N side. |
format | Text |
id | pubmed-2225896 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1969 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22258962008-04-23 Ion Transport and the Development of Hydrogen Ion Secretion in the Stomach of the Metamorphosing Bullfrog Tadpole Forte, John G. Limlomwongse, Liangchai Kasbekar, Dinkar K. J Gen Physiol Article Isolated bullfrog tadpole stomachs secrete H(+) by stage XXIV of metamorphosis, when tail reabsorption is nearly complete. At this stage the PD shows characteristic responses identical to those of the adult. The appearance of HCl secretion correlates well with other studies showing the morphogenesis of oxyntic cells. Prior to the development of H(+) secretion tadpole stomachs maintain a PD similar in polarity and magnitude to that of the adult; i.e., secretory (S) side negative with respect to the nutrient (N) side. The interdependence with aerobic metabolism appeared to increase progressively through metamorphosis; however, glycolytic inhibitors always abolished the PD. Isotopic flux analysis showed that the transepithelial movement of Na(+) was consistent with passive diffusion, whereas an active transport of Cl(-) from N to S was clearly indicated. Variations in [Na(+)], [K(+)], and [Cl(-)] in the bathing solutions induced changes consistent with the following functional description of the pre-H(+)-secreting tadpole stomach. (a) The S side is relatively permeable to Cl(-), but not to Na(+) or K(+). (b) An equilibrium potential for K(+) and Cl(-) exists at the N interface. (c) Ouabain abolishes the selective K(+) permeablity at the N interface and reduces the total PD. (d) Effects of Na(+) replacement by choline in the N solution become manifest only below 10–20 mM. It is concluded that prior to development of H(+) secretion, the tadpole gastric PD is generated by a Cl(-) pump from N to S and a Na(+) pump operating from the cell interior toward the N side. The Rockefeller University Press 1969-07-01 /pmc/articles/PMC2225896/ /pubmed/5792366 Text en Copyright © 1969 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Forte, John G. Limlomwongse, Liangchai Kasbekar, Dinkar K. Ion Transport and the Development of Hydrogen Ion Secretion in the Stomach of the Metamorphosing Bullfrog Tadpole |
title | Ion Transport and the Development of Hydrogen Ion Secretion in the Stomach of the Metamorphosing Bullfrog Tadpole |
title_full | Ion Transport and the Development of Hydrogen Ion Secretion in the Stomach of the Metamorphosing Bullfrog Tadpole |
title_fullStr | Ion Transport and the Development of Hydrogen Ion Secretion in the Stomach of the Metamorphosing Bullfrog Tadpole |
title_full_unstemmed | Ion Transport and the Development of Hydrogen Ion Secretion in the Stomach of the Metamorphosing Bullfrog Tadpole |
title_short | Ion Transport and the Development of Hydrogen Ion Secretion in the Stomach of the Metamorphosing Bullfrog Tadpole |
title_sort | ion transport and the development of hydrogen ion secretion in the stomach of the metamorphosing bullfrog tadpole |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225896/ https://www.ncbi.nlm.nih.gov/pubmed/5792366 |
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