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β-Amyloid 25-35 Peptide Reduces the Expression of Glutamine Transporter SAT1 in Cultured Cortical Neurons

β-Amyloid (Aβ) peptides may cause malfunction and death of neurons in Alzheimer’s disease. We investigated the effect of Aβ on key transporters of amino acid neurotransmission in cells cultured from rat cerebral cortex. The cultures were treated with Aβ(25-35) at 3 and 10 μM for 12 and 24 h followed...

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Autores principales: Buntup, Doungjai, Skare, Øivind, Solbu, Tom Tallak, Chaudhry, Farrukh A., Storm-Mathisen, Jon, Thangnipon, Wipawan
Formato: Texto
Lenguaje:English
Publicado: Springer US 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2226019/
https://www.ncbi.nlm.nih.gov/pubmed/18058230
http://dx.doi.org/10.1007/s11064-007-9527-2
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author Buntup, Doungjai
Skare, Øivind
Solbu, Tom Tallak
Chaudhry, Farrukh A.
Storm-Mathisen, Jon
Thangnipon, Wipawan
author_facet Buntup, Doungjai
Skare, Øivind
Solbu, Tom Tallak
Chaudhry, Farrukh A.
Storm-Mathisen, Jon
Thangnipon, Wipawan
author_sort Buntup, Doungjai
collection PubMed
description β-Amyloid (Aβ) peptides may cause malfunction and death of neurons in Alzheimer’s disease. We investigated the effect of Aβ on key transporters of amino acid neurotransmission in cells cultured from rat cerebral cortex. The cultures were treated with Aβ(25-35) at 3 and 10 μM for 12 and 24 h followed by quantitative analysis of immunofluorescence intensity. In mixed neuronal–glial cell cultures (from P1 rats), Aβ reduced the concentration of system A glutamine transporter 1 (SAT1), by up to 50% expressed relative to the neuronal marker microtubule-associated protein 2 (MAP2) in the same cell. No significant effects were detected on vesicular glutamate transporters VGLUT1 or VGLUT2 in neurons, or on glial system N glutamine transporter 1 (SN1). In neuronal cell cultures (from E18 rats), Aβ(25-35) did not reduce SAT1 immunoreactivity, suggesting that the observed effect depends on the presence of astroglia. The results indicate that Aβ may impair neuronal function and transmitter synthesis, and perhaps reduce excitotoxicity, through a reduction in neuronal glutamine uptake.
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spelling pubmed-22260192008-02-04 β-Amyloid 25-35 Peptide Reduces the Expression of Glutamine Transporter SAT1 in Cultured Cortical Neurons Buntup, Doungjai Skare, Øivind Solbu, Tom Tallak Chaudhry, Farrukh A. Storm-Mathisen, Jon Thangnipon, Wipawan Neurochem Res Original Paper β-Amyloid (Aβ) peptides may cause malfunction and death of neurons in Alzheimer’s disease. We investigated the effect of Aβ on key transporters of amino acid neurotransmission in cells cultured from rat cerebral cortex. The cultures were treated with Aβ(25-35) at 3 and 10 μM for 12 and 24 h followed by quantitative analysis of immunofluorescence intensity. In mixed neuronal–glial cell cultures (from P1 rats), Aβ reduced the concentration of system A glutamine transporter 1 (SAT1), by up to 50% expressed relative to the neuronal marker microtubule-associated protein 2 (MAP2) in the same cell. No significant effects were detected on vesicular glutamate transporters VGLUT1 or VGLUT2 in neurons, or on glial system N glutamine transporter 1 (SN1). In neuronal cell cultures (from E18 rats), Aβ(25-35) did not reduce SAT1 immunoreactivity, suggesting that the observed effect depends on the presence of astroglia. The results indicate that Aβ may impair neuronal function and transmitter synthesis, and perhaps reduce excitotoxicity, through a reduction in neuronal glutamine uptake. Springer US 2007-11-01 2008-02 /pmc/articles/PMC2226019/ /pubmed/18058230 http://dx.doi.org/10.1007/s11064-007-9527-2 Text en © Springer Science+Business Media, LLC 2007
spellingShingle Original Paper
Buntup, Doungjai
Skare, Øivind
Solbu, Tom Tallak
Chaudhry, Farrukh A.
Storm-Mathisen, Jon
Thangnipon, Wipawan
β-Amyloid 25-35 Peptide Reduces the Expression of Glutamine Transporter SAT1 in Cultured Cortical Neurons
title β-Amyloid 25-35 Peptide Reduces the Expression of Glutamine Transporter SAT1 in Cultured Cortical Neurons
title_full β-Amyloid 25-35 Peptide Reduces the Expression of Glutamine Transporter SAT1 in Cultured Cortical Neurons
title_fullStr β-Amyloid 25-35 Peptide Reduces the Expression of Glutamine Transporter SAT1 in Cultured Cortical Neurons
title_full_unstemmed β-Amyloid 25-35 Peptide Reduces the Expression of Glutamine Transporter SAT1 in Cultured Cortical Neurons
title_short β-Amyloid 25-35 Peptide Reduces the Expression of Glutamine Transporter SAT1 in Cultured Cortical Neurons
title_sort β-amyloid 25-35 peptide reduces the expression of glutamine transporter sat1 in cultured cortical neurons
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2226019/
https://www.ncbi.nlm.nih.gov/pubmed/18058230
http://dx.doi.org/10.1007/s11064-007-9527-2
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