Biochemical isolation and physiological identification of the egg- laying hormone in Aplysia californica

It has been determined that the bag cells of Aplysia californica produce two polypeptide species that comigrate on electrophoretic gels containing sodium dodecyl sulfate. By this separation procedure both species can be assigned a molecular weight of approximately 6,000. One of these molecules has a...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1976
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2228423/
https://www.ncbi.nlm.nih.gov/pubmed/956770
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collection PubMed
description It has been determined that the bag cells of Aplysia californica produce two polypeptide species that comigrate on electrophoretic gels containing sodium dodecyl sulfate. By this separation procedure both species can be assigned a molecular weight of approximately 6,000. One of these molecules has an Rf of 0.65 on alkaline discontinuous electrophoresis gels, an isoelectric point at pH 4.8, a gel filtration molecular weight of approximately 12,000, and has no known biological function. The other does not enter alkaline disk gels, has an isoelectric point at approximately pH 9.3, shows a gel filtration molecular weight consistent with that determined by SDS gel electrophoresis, and is the egg-laying hormone.
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spelling pubmed-22284232008-04-23 Biochemical isolation and physiological identification of the egg- laying hormone in Aplysia californica J Gen Physiol Articles It has been determined that the bag cells of Aplysia californica produce two polypeptide species that comigrate on electrophoretic gels containing sodium dodecyl sulfate. By this separation procedure both species can be assigned a molecular weight of approximately 6,000. One of these molecules has an Rf of 0.65 on alkaline discontinuous electrophoresis gels, an isoelectric point at pH 4.8, a gel filtration molecular weight of approximately 12,000, and has no known biological function. The other does not enter alkaline disk gels, has an isoelectric point at approximately pH 9.3, shows a gel filtration molecular weight consistent with that determined by SDS gel electrophoresis, and is the egg-laying hormone. The Rockefeller University Press 1976-08-01 /pmc/articles/PMC2228423/ /pubmed/956770 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Biochemical isolation and physiological identification of the egg- laying hormone in Aplysia californica
title Biochemical isolation and physiological identification of the egg- laying hormone in Aplysia californica
title_full Biochemical isolation and physiological identification of the egg- laying hormone in Aplysia californica
title_fullStr Biochemical isolation and physiological identification of the egg- laying hormone in Aplysia californica
title_full_unstemmed Biochemical isolation and physiological identification of the egg- laying hormone in Aplysia californica
title_short Biochemical isolation and physiological identification of the egg- laying hormone in Aplysia californica
title_sort biochemical isolation and physiological identification of the egg- laying hormone in aplysia californica
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2228423/
https://www.ncbi.nlm.nih.gov/pubmed/956770

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