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Radial propagation of muscle action potential along the tubular system examined by potential-sensitive dyes
Isolated single (Xenopus) muscle fibers were stained with a non-permeant potential-probing dye, merocyanine rhodanine (WW375) or merocyanine oxazolone (NK2367). When the fiber was massively stimulated, an absorption change (wave a), which seemed to reflect the action potential, occurred. Simultaneou...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1980
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2228607/ https://www.ncbi.nlm.nih.gov/pubmed/10822502 |
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author | Nakajima, S. Gilai, A. |
author_facet | Nakajima, S. Gilai, A. |
author_sort | Nakajima, S. |
collection | PubMed |
description | Isolated single (Xenopus) muscle fibers were stained with a non-permeant potential-probing dye, merocyanine rhodanine (WW375) or merocyanine oxazolone (NK2367). When the fiber was massively stimulated, an absorption change (wave a), which seemed to reflect the action potential, occurred. Simultaneous recording of optical changes and intracellular action potentials revealed that the time-course of wave a was slower than the action potential: the peak of wave a was attained at 1 ms, and the peak of action potential was reached at 0.5 ms after the stimulation. This difference suggests that wave a represents the potential changes of the whole tubular membrane and the surface membrane, whereas the action potential represents a surface potential change. This idea was substantiated by recording absorption signals preferentially from the surface membrane by recording the absorption changes at the edge of the fiber. Wave a obtained by this method was as quick as the intracellular action potential. The value of radial conduction velocity of action potential along the T system, calculated by comparing the action potential with wave a, was 6.4 cm/s at 24.5 degrees C, in fair agreement with Gonzalez-Serratos (1971. J. Physiol. [Lond.]. 212:777-799). The shape of wave a suggests the existence of an access delay (a conduction delay at the orifice of the T system) of 130 microseconds. |
format | Text |
id | pubmed-2228607 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1980 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22286072008-04-23 Radial propagation of muscle action potential along the tubular system examined by potential-sensitive dyes Nakajima, S. Gilai, A. J Gen Physiol Journal Article Isolated single (Xenopus) muscle fibers were stained with a non-permeant potential-probing dye, merocyanine rhodanine (WW375) or merocyanine oxazolone (NK2367). When the fiber was massively stimulated, an absorption change (wave a), which seemed to reflect the action potential, occurred. Simultaneous recording of optical changes and intracellular action potentials revealed that the time-course of wave a was slower than the action potential: the peak of wave a was attained at 1 ms, and the peak of action potential was reached at 0.5 ms after the stimulation. This difference suggests that wave a represents the potential changes of the whole tubular membrane and the surface membrane, whereas the action potential represents a surface potential change. This idea was substantiated by recording absorption signals preferentially from the surface membrane by recording the absorption changes at the edge of the fiber. Wave a obtained by this method was as quick as the intracellular action potential. The value of radial conduction velocity of action potential along the T system, calculated by comparing the action potential with wave a, was 6.4 cm/s at 24.5 degrees C, in fair agreement with Gonzalez-Serratos (1971. J. Physiol. [Lond.]. 212:777-799). The shape of wave a suggests the existence of an access delay (a conduction delay at the orifice of the T system) of 130 microseconds. The Rockefeller University Press 1980-12-01 /pmc/articles/PMC2228607/ /pubmed/10822502 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Journal Article Nakajima, S. Gilai, A. Radial propagation of muscle action potential along the tubular system examined by potential-sensitive dyes |
title | Radial propagation of muscle action potential along the tubular system examined by potential-sensitive dyes |
title_full | Radial propagation of muscle action potential along the tubular system examined by potential-sensitive dyes |
title_fullStr | Radial propagation of muscle action potential along the tubular system examined by potential-sensitive dyes |
title_full_unstemmed | Radial propagation of muscle action potential along the tubular system examined by potential-sensitive dyes |
title_short | Radial propagation of muscle action potential along the tubular system examined by potential-sensitive dyes |
title_sort | radial propagation of muscle action potential along the tubular system examined by potential-sensitive dyes |
topic | Journal Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2228607/ https://www.ncbi.nlm.nih.gov/pubmed/10822502 |
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