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Patch recordings from the electrocytes of electrophorus. Na channel gating currents
Gating currents were recorded at 11 degrees C in cell-attached and inside-out patches from the innervated membrane of Electrophorus main organ electrocytes. With pipette tip diameters of 3-8 microns, maximal charge measured in patches ranged from 0.74 to 7.19 fC. The general features of the gating c...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1991
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2229061/ https://www.ncbi.nlm.nih.gov/pubmed/1662259 |
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collection | PubMed |
description | Gating currents were recorded at 11 degrees C in cell-attached and inside-out patches from the innervated membrane of Electrophorus main organ electrocytes. With pipette tip diameters of 3-8 microns, maximal charge measured in patches ranged from 0.74 to 7.19 fC. The general features of the gating currents are similar to those from the squid giant axon. The steady-state voltage dependence of the ON gating charge was characterized by an effective valence of 1.3 +/- 0.4 and a midpoint voltage of -56 +/- 7 mV. The charge vs. voltage relation lies approximately 30 mV negative to the channel open probability curve. The ratio of the time constants of the OFF gating current and the Na current was 2.3 at -120 mV and equal at -80 mV. Charge immobilization and Na current inactivation develop with comparable time courses and have very similar voltage dependences. Between 60 and 80% of the charge is temporarily immobilized by inactivation. |
format | Text |
id | pubmed-2229061 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1991 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22290612008-04-23 Patch recordings from the electrocytes of electrophorus. Na channel gating currents J Gen Physiol Articles Gating currents were recorded at 11 degrees C in cell-attached and inside-out patches from the innervated membrane of Electrophorus main organ electrocytes. With pipette tip diameters of 3-8 microns, maximal charge measured in patches ranged from 0.74 to 7.19 fC. The general features of the gating currents are similar to those from the squid giant axon. The steady-state voltage dependence of the ON gating charge was characterized by an effective valence of 1.3 +/- 0.4 and a midpoint voltage of -56 +/- 7 mV. The charge vs. voltage relation lies approximately 30 mV negative to the channel open probability curve. The ratio of the time constants of the OFF gating current and the Na current was 2.3 at -120 mV and equal at -80 mV. Charge immobilization and Na current inactivation develop with comparable time courses and have very similar voltage dependences. Between 60 and 80% of the charge is temporarily immobilized by inactivation. The Rockefeller University Press 1991-09-01 /pmc/articles/PMC2229061/ /pubmed/1662259 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Patch recordings from the electrocytes of electrophorus. Na channel gating currents |
title | Patch recordings from the electrocytes of electrophorus. Na channel gating currents |
title_full | Patch recordings from the electrocytes of electrophorus. Na channel gating currents |
title_fullStr | Patch recordings from the electrocytes of electrophorus. Na channel gating currents |
title_full_unstemmed | Patch recordings from the electrocytes of electrophorus. Na channel gating currents |
title_short | Patch recordings from the electrocytes of electrophorus. Na channel gating currents |
title_sort | patch recordings from the electrocytes of electrophorus. na channel gating currents |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2229061/ https://www.ncbi.nlm.nih.gov/pubmed/1662259 |