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A Structural Rearrangement in the Sodium Channel Pore Linked to Slow Inactivation and Use Dependence

Voltage-gated sodium (Na(+)) channels are a fundamental target for modulating excitability in neuronal and muscle cells. When depolarized, Na(+) channels may gradually enter long-lived, slow-inactivated conformational states, causing a cumulative loss of function. Although the structural motifs that...

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Autores principales: Ong, Boon-Hooi, Tomaselli, Gordon F., Balser, Jeffrey R.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2229478/
https://www.ncbi.nlm.nih.gov/pubmed/11055994
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author Ong, Boon-Hooi
Tomaselli, Gordon F.
Balser, Jeffrey R.
author_facet Ong, Boon-Hooi
Tomaselli, Gordon F.
Balser, Jeffrey R.
author_sort Ong, Boon-Hooi
collection PubMed
description Voltage-gated sodium (Na(+)) channels are a fundamental target for modulating excitability in neuronal and muscle cells. When depolarized, Na(+) channels may gradually enter long-lived, slow-inactivated conformational states, causing a cumulative loss of function. Although the structural motifs that underlie transient, depolarization-induced Na(+) channel conformational states are increasingly recognized, the conformational changes responsible for more sustained forms of inactivation are unresolved. Recent studies have shown that slow inactivation components exhibiting a range of kinetic behavior (from tens of milliseconds to seconds) are modified by mutations in the outer pore P-segments. We examined the state-dependent accessibility of an engineered cysteine in the domain III, P-segment (F1236C; rat skeletal muscle) to methanethiosulfonate-ethylammonium (MTSEA) using whole-cell current recordings in HEK 293 cells. F1236C was reactive with MTSEA applied from outside, but not inside the cell, and modification was markedly increased by depolarization. Depolarized F1236C channels exhibited both intermediate (I(M); τ ∼ 30 ms) and slower (I(S); τ ∼ 2 s) kinetic components of slow inactivation. Trains of brief, 5-ms depolarizations, which did not induce slow inactivation, produced more rapid modification than did longer (100 ms or 6 s) pulse widths, suggesting both the I(M) and I(S) kinetic components inhibit depolarization-induced MTSEA accessibility of the cysteine side chain. Lidocaine inhibited the depolarization-dependent sulfhydryl modification induced by sustained (100 ms) depolarizations, but not by brief (5 ms) depolarizations. We conclude that competing forces influence the depolarization-dependent modification of the cysteine side chain: conformational changes associated with brief periods of depolarization enhance accessibility, whereas slow inactivation tends to inhibit the side chain accessibility. The findings suggest that slow Na(+) channel inactivation and use-dependent lidocaine action are linked to a structural rearrangement in the outer pore.
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spelling pubmed-22294782008-04-21 A Structural Rearrangement in the Sodium Channel Pore Linked to Slow Inactivation and Use Dependence Ong, Boon-Hooi Tomaselli, Gordon F. Balser, Jeffrey R. J Gen Physiol Original Article Voltage-gated sodium (Na(+)) channels are a fundamental target for modulating excitability in neuronal and muscle cells. When depolarized, Na(+) channels may gradually enter long-lived, slow-inactivated conformational states, causing a cumulative loss of function. Although the structural motifs that underlie transient, depolarization-induced Na(+) channel conformational states are increasingly recognized, the conformational changes responsible for more sustained forms of inactivation are unresolved. Recent studies have shown that slow inactivation components exhibiting a range of kinetic behavior (from tens of milliseconds to seconds) are modified by mutations in the outer pore P-segments. We examined the state-dependent accessibility of an engineered cysteine in the domain III, P-segment (F1236C; rat skeletal muscle) to methanethiosulfonate-ethylammonium (MTSEA) using whole-cell current recordings in HEK 293 cells. F1236C was reactive with MTSEA applied from outside, but not inside the cell, and modification was markedly increased by depolarization. Depolarized F1236C channels exhibited both intermediate (I(M); τ ∼ 30 ms) and slower (I(S); τ ∼ 2 s) kinetic components of slow inactivation. Trains of brief, 5-ms depolarizations, which did not induce slow inactivation, produced more rapid modification than did longer (100 ms or 6 s) pulse widths, suggesting both the I(M) and I(S) kinetic components inhibit depolarization-induced MTSEA accessibility of the cysteine side chain. Lidocaine inhibited the depolarization-dependent sulfhydryl modification induced by sustained (100 ms) depolarizations, but not by brief (5 ms) depolarizations. We conclude that competing forces influence the depolarization-dependent modification of the cysteine side chain: conformational changes associated with brief periods of depolarization enhance accessibility, whereas slow inactivation tends to inhibit the side chain accessibility. The findings suggest that slow Na(+) channel inactivation and use-dependent lidocaine action are linked to a structural rearrangement in the outer pore. The Rockefeller University Press 2000-11-01 /pmc/articles/PMC2229478/ /pubmed/11055994 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Ong, Boon-Hooi
Tomaselli, Gordon F.
Balser, Jeffrey R.
A Structural Rearrangement in the Sodium Channel Pore Linked to Slow Inactivation and Use Dependence
title A Structural Rearrangement in the Sodium Channel Pore Linked to Slow Inactivation and Use Dependence
title_full A Structural Rearrangement in the Sodium Channel Pore Linked to Slow Inactivation and Use Dependence
title_fullStr A Structural Rearrangement in the Sodium Channel Pore Linked to Slow Inactivation and Use Dependence
title_full_unstemmed A Structural Rearrangement in the Sodium Channel Pore Linked to Slow Inactivation and Use Dependence
title_short A Structural Rearrangement in the Sodium Channel Pore Linked to Slow Inactivation and Use Dependence
title_sort structural rearrangement in the sodium channel pore linked to slow inactivation and use dependence
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2229478/
https://www.ncbi.nlm.nih.gov/pubmed/11055994
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