Na(+) Block and Permeation in a K(+) Channel of Known Structure

The effects of intracellular Na(+) were studied on K(+) and Rb(+) currents through single KcsA channels. At low voltage, Na(+) produces voltage-dependent block, which becomes relieved at high voltage by a “punchthrough” mechanism representing Na(+) escaping from its blocking site through the selecti...

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Detalles Bibliográficos
Autores principales: Nimigean, Crina M., Miller, Christopher
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2002
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2229518/
https://www.ncbi.nlm.nih.gov/pubmed/12198089
http://dx.doi.org/10.1085/jgp.20028614
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author Nimigean, Crina M.
Miller, Christopher
author_facet Nimigean, Crina M.
Miller, Christopher
author_sort Nimigean, Crina M.
collection PubMed
description The effects of intracellular Na(+) were studied on K(+) and Rb(+) currents through single KcsA channels. At low voltage, Na(+) produces voltage-dependent block, which becomes relieved at high voltage by a “punchthrough” mechanism representing Na(+) escaping from its blocking site through the selectivity filter. The Na(+) blocking site is located in the wide, hydrated vestibule, and it displays unexpected selectivity for K(+) and Rb(+) against Na(+). The voltage dependence of Na(+) block reflects coordinated movements of the blocker with permeant ions in the selectivity filter.
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spelling pubmed-22295182008-04-16 Na(+) Block and Permeation in a K(+) Channel of Known Structure Nimigean, Crina M. Miller, Christopher J Gen Physiol Article The effects of intracellular Na(+) were studied on K(+) and Rb(+) currents through single KcsA channels. At low voltage, Na(+) produces voltage-dependent block, which becomes relieved at high voltage by a “punchthrough” mechanism representing Na(+) escaping from its blocking site through the selectivity filter. The Na(+) blocking site is located in the wide, hydrated vestibule, and it displays unexpected selectivity for K(+) and Rb(+) against Na(+). The voltage dependence of Na(+) block reflects coordinated movements of the blocker with permeant ions in the selectivity filter. The Rockefeller University Press 2002-09 /pmc/articles/PMC2229518/ /pubmed/12198089 http://dx.doi.org/10.1085/jgp.20028614 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Nimigean, Crina M.
Miller, Christopher
Na(+) Block and Permeation in a K(+) Channel of Known Structure
title Na(+) Block and Permeation in a K(+) Channel of Known Structure
title_full Na(+) Block and Permeation in a K(+) Channel of Known Structure
title_fullStr Na(+) Block and Permeation in a K(+) Channel of Known Structure
title_full_unstemmed Na(+) Block and Permeation in a K(+) Channel of Known Structure
title_short Na(+) Block and Permeation in a K(+) Channel of Known Structure
title_sort na(+) block and permeation in a k(+) channel of known structure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2229518/
https://www.ncbi.nlm.nih.gov/pubmed/12198089
http://dx.doi.org/10.1085/jgp.20028614
work_keys_str_mv AT nimigeancrinam nablockandpermeationinakchannelofknownstructure
AT millerchristopher nablockandpermeationinakchannelofknownstructure

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