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Responses of Xenopus laevis Water Nose to Water-Soluble and Volatile Odorants

Using the whole-cell mode of the patch-clamp technique, we recorded action potentials, voltage-activated cationic currents, and inward currents in response to water-soluble and volatile odorants from receptor neurons in the lateral diverticulum (water nose) of the olfactory sensory epithelium of Xen...

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Detalles Bibliográficos
Autores principales: Iida, Akio, Kashiwayanagi, Makoto
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2229635/
https://www.ncbi.nlm.nih.gov/pubmed/10398694
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author Iida, Akio
Kashiwayanagi, Makoto
author_facet Iida, Akio
Kashiwayanagi, Makoto
author_sort Iida, Akio
collection PubMed
description Using the whole-cell mode of the patch-clamp technique, we recorded action potentials, voltage-activated cationic currents, and inward currents in response to water-soluble and volatile odorants from receptor neurons in the lateral diverticulum (water nose) of the olfactory sensory epithelium of Xenopus laevis. The resting membrane potential was −46.5 ± 1.2 mV [Formula: see text] , and a current injection of 1–3 pA induced overshooting action potentials. Under voltage-clamp conditions, a voltage-dependent Na(+) inward current, a sustained outward K(+) current, and a Ca(2+)-activated K(+) current were identified. Application of an amino acid cocktail induced inward currents in 32 of 238 olfactory neurons in the lateral diverticulum under voltage-clamp conditions. Application of volatile odorant cocktails also induced current responses in 23 of 238 olfactory neurons. These results suggest that the olfactory neurons respond to both water-soluble and volatile odorants. The application of alanine or arginine induced inward currents in a dose-dependent manner. More than 50% of the single olfactory neurons responded to multiple types of amino acids, including acidic, neutral, and basic amino acids applied at 100 μM or 1 mM. These results suggest that olfactory neurons in the lateral diverticulum have receptors for amino acids and volatile odorants.
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spelling pubmed-22296352008-04-22 Responses of Xenopus laevis Water Nose to Water-Soluble and Volatile Odorants Iida, Akio Kashiwayanagi, Makoto J Gen Physiol Original Article Using the whole-cell mode of the patch-clamp technique, we recorded action potentials, voltage-activated cationic currents, and inward currents in response to water-soluble and volatile odorants from receptor neurons in the lateral diverticulum (water nose) of the olfactory sensory epithelium of Xenopus laevis. The resting membrane potential was −46.5 ± 1.2 mV [Formula: see text] , and a current injection of 1–3 pA induced overshooting action potentials. Under voltage-clamp conditions, a voltage-dependent Na(+) inward current, a sustained outward K(+) current, and a Ca(2+)-activated K(+) current were identified. Application of an amino acid cocktail induced inward currents in 32 of 238 olfactory neurons in the lateral diverticulum under voltage-clamp conditions. Application of volatile odorant cocktails also induced current responses in 23 of 238 olfactory neurons. These results suggest that the olfactory neurons respond to both water-soluble and volatile odorants. The application of alanine or arginine induced inward currents in a dose-dependent manner. More than 50% of the single olfactory neurons responded to multiple types of amino acids, including acidic, neutral, and basic amino acids applied at 100 μM or 1 mM. These results suggest that olfactory neurons in the lateral diverticulum have receptors for amino acids and volatile odorants. The Rockefeller University Press 1999-07-01 /pmc/articles/PMC2229635/ /pubmed/10398694 Text en © 1999 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Iida, Akio
Kashiwayanagi, Makoto
Responses of Xenopus laevis Water Nose to Water-Soluble and Volatile Odorants
title Responses of Xenopus laevis Water Nose to Water-Soluble and Volatile Odorants
title_full Responses of Xenopus laevis Water Nose to Water-Soluble and Volatile Odorants
title_fullStr Responses of Xenopus laevis Water Nose to Water-Soluble and Volatile Odorants
title_full_unstemmed Responses of Xenopus laevis Water Nose to Water-Soluble and Volatile Odorants
title_short Responses of Xenopus laevis Water Nose to Water-Soluble and Volatile Odorants
title_sort responses of xenopus laevis water nose to water-soluble and volatile odorants
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2229635/
https://www.ncbi.nlm.nih.gov/pubmed/10398694
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