BUFFERED OSMIUM TETROXIDE (OsO(4)) FIXATION FOR CYTOLOGICAL AND FEULGEN MICROSPECTROPHOTOMETRIC STUDIES OF HUMAN RECTAL POLYPS

Evidence has been presented that when human rectal mucosa and polyps are fixed with buffered OsO(4) according to the method of Palade, staining of paraffin sections by basic and acidic dyes results in a distinct color difference between chromatin and cytoplasm. Furthermore, the Feulgen reaction also...

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Detalles Bibliográficos
Autores principales: Leuchtenberger, Cecilie, Doolin, Paul F., Kutsakis, Anne H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1955
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2229658/
https://www.ncbi.nlm.nih.gov/pubmed/13263327
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author Leuchtenberger, Cecilie
Doolin, Paul F.
Kutsakis, Anne H.
author_facet Leuchtenberger, Cecilie
Doolin, Paul F.
Kutsakis, Anne H.
author_sort Leuchtenberger, Cecilie
collection PubMed
description Evidence has been presented that when human rectal mucosa and polyps are fixed with buffered OsO(4) according to the method of Palade, staining of paraffin sections by basic and acidic dyes results in a distinct color difference between chromatin and cytoplasm. Furthermore, the Feulgen reaction also can be carried out successfully and such OsO(4)-fixed Feulgen-stained sections are especially suitable for microspectrophotometric determinations of DNA in individual nuclei.
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spelling pubmed-22296582008-05-01 BUFFERED OSMIUM TETROXIDE (OsO(4)) FIXATION FOR CYTOLOGICAL AND FEULGEN MICROSPECTROPHOTOMETRIC STUDIES OF HUMAN RECTAL POLYPS Leuchtenberger, Cecilie Doolin, Paul F. Kutsakis, Anne H. J Biophys Biochem Cytol Article Evidence has been presented that when human rectal mucosa and polyps are fixed with buffered OsO(4) according to the method of Palade, staining of paraffin sections by basic and acidic dyes results in a distinct color difference between chromatin and cytoplasm. Furthermore, the Feulgen reaction also can be carried out successfully and such OsO(4)-fixed Feulgen-stained sections are especially suitable for microspectrophotometric determinations of DNA in individual nuclei. The Rockefeller University Press 1955-09-25 /pmc/articles/PMC2229658/ /pubmed/13263327 Text en Copyright © Copyright, 1955, by The Rockefeller Institute for Medical Research
spellingShingle Article
Leuchtenberger, Cecilie
Doolin, Paul F.
Kutsakis, Anne H.
BUFFERED OSMIUM TETROXIDE (OsO(4)) FIXATION FOR CYTOLOGICAL AND FEULGEN MICROSPECTROPHOTOMETRIC STUDIES OF HUMAN RECTAL POLYPS
title BUFFERED OSMIUM TETROXIDE (OsO(4)) FIXATION FOR CYTOLOGICAL AND FEULGEN MICROSPECTROPHOTOMETRIC STUDIES OF HUMAN RECTAL POLYPS
title_full BUFFERED OSMIUM TETROXIDE (OsO(4)) FIXATION FOR CYTOLOGICAL AND FEULGEN MICROSPECTROPHOTOMETRIC STUDIES OF HUMAN RECTAL POLYPS
title_fullStr BUFFERED OSMIUM TETROXIDE (OsO(4)) FIXATION FOR CYTOLOGICAL AND FEULGEN MICROSPECTROPHOTOMETRIC STUDIES OF HUMAN RECTAL POLYPS
title_full_unstemmed BUFFERED OSMIUM TETROXIDE (OsO(4)) FIXATION FOR CYTOLOGICAL AND FEULGEN MICROSPECTROPHOTOMETRIC STUDIES OF HUMAN RECTAL POLYPS
title_short BUFFERED OSMIUM TETROXIDE (OsO(4)) FIXATION FOR CYTOLOGICAL AND FEULGEN MICROSPECTROPHOTOMETRIC STUDIES OF HUMAN RECTAL POLYPS
title_sort buffered osmium tetroxide (oso(4)) fixation for cytological and feulgen microspectrophotometric studies of human rectal polyps
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2229658/
https://www.ncbi.nlm.nih.gov/pubmed/13263327
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AT kutsakisanneh bufferedosmiumtetroxideoso4fixationforcytologicalandfeulgenmicrospectrophotometricstudiesofhumanrectalpolyps

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