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ON ARTIFACTS APPEARING IN THE HISTOCHEMICAL FIXATION OF GLYCOGEN

1. Fixation artifacts associated with glycogen translocation are prevalent in tissues of parenchymatous type and scarce or non-existent in tissues of loose type. 2. Liver tissue treated with M/3 NaOH solution before fixation did not show an uneven distribution of glycogen. This was interpreted as in...

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Detalles Bibliográficos
Autores principales: Takahashi, Kohnosuke, Iwase, Shoji
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1955
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2229660/
https://www.ncbi.nlm.nih.gov/pubmed/13263328
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author Takahashi, Kohnosuke
Iwase, Shoji
author_facet Takahashi, Kohnosuke
Iwase, Shoji
author_sort Takahashi, Kohnosuke
collection PubMed
description 1. Fixation artifacts associated with glycogen translocation are prevalent in tissues of parenchymatous type and scarce or non-existent in tissues of loose type. 2. Liver tissue treated with M/3 NaOH solution before fixation did not show an uneven distribution of glycogen. This was interpreted as indicating that the liver, a tissue of parenchymatous type, was changed, so to speak, into a loose type of tissue by alkali treatment. 3. The so called Alkohol-flucht of glycogen was produced in Yoshida's ascites tumor cells by a procedure which changed a loose type of tissue into a parenchymatous one, that is, by packing the tumor cells tightly. 4. The translocation of glycogen in cells appeared to occur when the fixatives penetrated the cells rapidly from a single direction, but failed to occur when the cells were attacked by the fixative from all directions. 5. In dried smears of Yoshida's ascites tumor cells and bone marrow cells, the glycogen particles are translocated to the peripheral regions of the cells, and coalesce there. The production of these artifacts is related in some way to the physicochemical properties of the protoplasm and plasma membrane of the cells.
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spelling pubmed-22296602008-05-01 ON ARTIFACTS APPEARING IN THE HISTOCHEMICAL FIXATION OF GLYCOGEN Takahashi, Kohnosuke Iwase, Shoji J Biophys Biochem Cytol Article 1. Fixation artifacts associated with glycogen translocation are prevalent in tissues of parenchymatous type and scarce or non-existent in tissues of loose type. 2. Liver tissue treated with M/3 NaOH solution before fixation did not show an uneven distribution of glycogen. This was interpreted as indicating that the liver, a tissue of parenchymatous type, was changed, so to speak, into a loose type of tissue by alkali treatment. 3. The so called Alkohol-flucht of glycogen was produced in Yoshida's ascites tumor cells by a procedure which changed a loose type of tissue into a parenchymatous one, that is, by packing the tumor cells tightly. 4. The translocation of glycogen in cells appeared to occur when the fixatives penetrated the cells rapidly from a single direction, but failed to occur when the cells were attacked by the fixative from all directions. 5. In dried smears of Yoshida's ascites tumor cells and bone marrow cells, the glycogen particles are translocated to the peripheral regions of the cells, and coalesce there. The production of these artifacts is related in some way to the physicochemical properties of the protoplasm and plasma membrane of the cells. The Rockefeller University Press 1955-09-25 /pmc/articles/PMC2229660/ /pubmed/13263328 Text en Copyright © Copyright, 1955, by The Rockefeller Institute for Medical Research
spellingShingle Article
Takahashi, Kohnosuke
Iwase, Shoji
ON ARTIFACTS APPEARING IN THE HISTOCHEMICAL FIXATION OF GLYCOGEN
title ON ARTIFACTS APPEARING IN THE HISTOCHEMICAL FIXATION OF GLYCOGEN
title_full ON ARTIFACTS APPEARING IN THE HISTOCHEMICAL FIXATION OF GLYCOGEN
title_fullStr ON ARTIFACTS APPEARING IN THE HISTOCHEMICAL FIXATION OF GLYCOGEN
title_full_unstemmed ON ARTIFACTS APPEARING IN THE HISTOCHEMICAL FIXATION OF GLYCOGEN
title_short ON ARTIFACTS APPEARING IN THE HISTOCHEMICAL FIXATION OF GLYCOGEN
title_sort on artifacts appearing in the histochemical fixation of glycogen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2229660/
https://www.ncbi.nlm.nih.gov/pubmed/13263328
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