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Basic Proteins of Plant Nuclei during Normal and Pathological Cell Growth

Histone proteins were studied by microphotometry of plant tissue sections stained with fast green at pH 8.1. For comparative purposes the Feulgen reaction was used for deoxyribose nuclei acid (DNA); the Sakaguchi reaction for arginine; and the Millon reaction for estimates of total protein. Analysis...

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Detalles Bibliográficos
Autores principales: Rasch, Ellen, Woodard, John W.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1959
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2229794/
https://www.ncbi.nlm.nih.gov/pubmed/14436319
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author Rasch, Ellen
Woodard, John W.
author_facet Rasch, Ellen
Woodard, John W.
author_sort Rasch, Ellen
collection PubMed
description Histone proteins were studied by microphotometry of plant tissue sections stained with fast green at pH 8.1. For comparative purposes the Feulgen reaction was used for deoxyribose nuclei acid (DNA); the Sakaguchi reaction for arginine; and the Millon reaction for estimates of total protein. Analysis of Tradescantia tissues indicated that amounts of nuclear histone fell into approximate multiples of the gametic (egg or sperm) quantity except in dividing tissues, where amounts intermediate between multiples were found. In differentiated tissues of lily, corn, onion, and broad bean, histones occurred in constant amounts per nucleus, characteristic of the species, as was found also for DNA. Unlike the condition in several animal species, the basic proteins of sperm nuclei in these higher plants were of the histone type; no evidence of protamine was found. In a plant neoplasm, crown gall of broad bean, behavior of the basic nuclear proteins closely paralleled that of DNA. Thus, alterations of DNA levels in tumor tissues were accompanied by quantitatively similar changes in histone levels to maintain the same Feulgen/fast green ratios found in homologous normal tissues.
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spelling pubmed-22297942008-05-01 Basic Proteins of Plant Nuclei during Normal and Pathological Cell Growth Rasch, Ellen Woodard, John W. J Biophys Biochem Cytol Article Histone proteins were studied by microphotometry of plant tissue sections stained with fast green at pH 8.1. For comparative purposes the Feulgen reaction was used for deoxyribose nuclei acid (DNA); the Sakaguchi reaction for arginine; and the Millon reaction for estimates of total protein. Analysis of Tradescantia tissues indicated that amounts of nuclear histone fell into approximate multiples of the gametic (egg or sperm) quantity except in dividing tissues, where amounts intermediate between multiples were found. In differentiated tissues of lily, corn, onion, and broad bean, histones occurred in constant amounts per nucleus, characteristic of the species, as was found also for DNA. Unlike the condition in several animal species, the basic proteins of sperm nuclei in these higher plants were of the histone type; no evidence of protamine was found. In a plant neoplasm, crown gall of broad bean, behavior of the basic nuclear proteins closely paralleled that of DNA. Thus, alterations of DNA levels in tumor tissues were accompanied by quantitatively similar changes in histone levels to maintain the same Feulgen/fast green ratios found in homologous normal tissues. The Rockefeller University Press 1959-10-01 /pmc/articles/PMC2229794/ /pubmed/14436319 Text en Copyright © Copyright, 1959, by The Rockefeller Institute
spellingShingle Article
Rasch, Ellen
Woodard, John W.
Basic Proteins of Plant Nuclei during Normal and Pathological Cell Growth
title Basic Proteins of Plant Nuclei during Normal and Pathological Cell Growth
title_full Basic Proteins of Plant Nuclei during Normal and Pathological Cell Growth
title_fullStr Basic Proteins of Plant Nuclei during Normal and Pathological Cell Growth
title_full_unstemmed Basic Proteins of Plant Nuclei during Normal and Pathological Cell Growth
title_short Basic Proteins of Plant Nuclei during Normal and Pathological Cell Growth
title_sort basic proteins of plant nuclei during normal and pathological cell growth
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2229794/
https://www.ncbi.nlm.nih.gov/pubmed/14436319
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AT woodardjohnw basicproteinsofplantnucleiduringnormalandpathologicalcellgrowth