Electron Microscope Observations on the Behavior of the Bacterial Cytoplasmic Membrane During Cellular Division

Bacterial cells were fixed in OsO(4), washed, dehydrated, and embedded in a methacrylate mixture. Ultrathin sections were cut on a Porter-Blum ultramicrotome and were examined in an RCA electron microscope, type EMU-2D. The sections revealed that the cytoplasmic membrane undergoes a centripetal grow...

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Detalles Bibliográficos
Autor principal: Chapman, George B.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1959
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2229804/
https://www.ncbi.nlm.nih.gov/pubmed/13809225
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author Chapman, George B.
author_facet Chapman, George B.
author_sort Chapman, George B.
collection PubMed
description Bacterial cells were fixed in OsO(4), washed, dehydrated, and embedded in a methacrylate mixture. Ultrathin sections were cut on a Porter-Blum ultramicrotome and were examined in an RCA electron microscope, type EMU-2D. The sections revealed that the cytoplasmic membrane undergoes a centripetal growth to form a membrane septum. This septum is formed as a double structure. Constriction of the daughter cells and deposition of cell wall material lead to the separation of the daughter cells. The bacterial cytoplasm appears to consist largely of 200 A granules and occasionally reveals arrays of parallel dense lines.
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spelling pubmed-22298042008-05-01 Electron Microscope Observations on the Behavior of the Bacterial Cytoplasmic Membrane During Cellular Division Chapman, George B. J Biophys Biochem Cytol Article Bacterial cells were fixed in OsO(4), washed, dehydrated, and embedded in a methacrylate mixture. Ultrathin sections were cut on a Porter-Blum ultramicrotome and were examined in an RCA electron microscope, type EMU-2D. The sections revealed that the cytoplasmic membrane undergoes a centripetal growth to form a membrane septum. This septum is formed as a double structure. Constriction of the daughter cells and deposition of cell wall material lead to the separation of the daughter cells. The bacterial cytoplasm appears to consist largely of 200 A granules and occasionally reveals arrays of parallel dense lines. The Rockefeller University Press 1959-10-01 /pmc/articles/PMC2229804/ /pubmed/13809225 Text en Copyright © Copyright, 1959, by The Rockefeller Institute
spellingShingle Article
Chapman, George B.
Electron Microscope Observations on the Behavior of the Bacterial Cytoplasmic Membrane During Cellular Division
title Electron Microscope Observations on the Behavior of the Bacterial Cytoplasmic Membrane During Cellular Division
title_full Electron Microscope Observations on the Behavior of the Bacterial Cytoplasmic Membrane During Cellular Division
title_fullStr Electron Microscope Observations on the Behavior of the Bacterial Cytoplasmic Membrane During Cellular Division
title_full_unstemmed Electron Microscope Observations on the Behavior of the Bacterial Cytoplasmic Membrane During Cellular Division
title_short Electron Microscope Observations on the Behavior of the Bacterial Cytoplasmic Membrane During Cellular Division
title_sort electron microscope observations on the behavior of the bacterial cytoplasmic membrane during cellular division
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2229804/
https://www.ncbi.nlm.nih.gov/pubmed/13809225
work_keys_str_mv AT chapmangeorgeb electronmicroscopeobservationsonthebehaviorofthebacterialcytoplasmicmembraneduringcellulardivision

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