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Dual Regulation of Calcium Mobilization by Inositol 1,4,5-Trisphosphate in a Living Cell

Changes in cytosolic free calcium ([Ca(2+)](i)) often take the form of a sustained response or repetitive oscillations. The frequency and amplitude of [Ca(2+)](i) oscillations are essential for the selective stimulation of gene expression and for enzyme activation. However, the mechanism that determ...

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Detalles Bibliográficos
Autores principales: Tertyshnikova, Svetlana, Fein, Alan
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2233754/
https://www.ncbi.nlm.nih.gov/pubmed/10736314
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author Tertyshnikova, Svetlana
Fein, Alan
author_facet Tertyshnikova, Svetlana
Fein, Alan
author_sort Tertyshnikova, Svetlana
collection PubMed
description Changes in cytosolic free calcium ([Ca(2+)](i)) often take the form of a sustained response or repetitive oscillations. The frequency and amplitude of [Ca(2+)](i) oscillations are essential for the selective stimulation of gene expression and for enzyme activation. However, the mechanism that determines whether [Ca(2+)](i) oscillates at a particular frequency or becomes a sustained response is poorly understood. We find that [Ca(2+)](i) oscillations in rat megakaryocytes, as in other cells, results from a Ca(2+)-dependent inhibition of inositol 1,4,5-trisphosphate (IP(3))–induced Ca(2+) release. Moreover, we find that this inhibition becomes progressively less effective with higher IP(3) concentrations. We suggest that disinhibition, by increasing IP(3) concentration, of Ca(2+)-dependent inhibition is a common mechanism for the regulation of [Ca(2+)](i) oscillations in cells containing IP(3)-sensitive Ca(2+) stores.
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spelling pubmed-22337542008-04-22 Dual Regulation of Calcium Mobilization by Inositol 1,4,5-Trisphosphate in a Living Cell Tertyshnikova, Svetlana Fein, Alan J Gen Physiol Original Article Changes in cytosolic free calcium ([Ca(2+)](i)) often take the form of a sustained response or repetitive oscillations. The frequency and amplitude of [Ca(2+)](i) oscillations are essential for the selective stimulation of gene expression and for enzyme activation. However, the mechanism that determines whether [Ca(2+)](i) oscillates at a particular frequency or becomes a sustained response is poorly understood. We find that [Ca(2+)](i) oscillations in rat megakaryocytes, as in other cells, results from a Ca(2+)-dependent inhibition of inositol 1,4,5-trisphosphate (IP(3))–induced Ca(2+) release. Moreover, we find that this inhibition becomes progressively less effective with higher IP(3) concentrations. We suggest that disinhibition, by increasing IP(3) concentration, of Ca(2+)-dependent inhibition is a common mechanism for the regulation of [Ca(2+)](i) oscillations in cells containing IP(3)-sensitive Ca(2+) stores. The Rockefeller University Press 2000-04-01 /pmc/articles/PMC2233754/ /pubmed/10736314 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Tertyshnikova, Svetlana
Fein, Alan
Dual Regulation of Calcium Mobilization by Inositol 1,4,5-Trisphosphate in a Living Cell
title Dual Regulation of Calcium Mobilization by Inositol 1,4,5-Trisphosphate in a Living Cell
title_full Dual Regulation of Calcium Mobilization by Inositol 1,4,5-Trisphosphate in a Living Cell
title_fullStr Dual Regulation of Calcium Mobilization by Inositol 1,4,5-Trisphosphate in a Living Cell
title_full_unstemmed Dual Regulation of Calcium Mobilization by Inositol 1,4,5-Trisphosphate in a Living Cell
title_short Dual Regulation of Calcium Mobilization by Inositol 1,4,5-Trisphosphate in a Living Cell
title_sort dual regulation of calcium mobilization by inositol 1,4,5-trisphosphate in a living cell
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2233754/
https://www.ncbi.nlm.nih.gov/pubmed/10736314
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