Cargando…

Stable hZW10 kinetochore residency, mediated by hZwint-1 interaction, is essential for the mitotic checkpoint

The mitotic checkpoint is an essential surveillance mechanism that ensures high fidelity chromosome segregation during mitosis. Mitotic checkpoint function depends on numerous kinetochore proteins, including ZW10, ROD, and Zwilch (the ROD–ZW10–Zwilch complex). Through an extensive mutagenesis screen...

Descripción completa

Detalles Bibliográficos
Autores principales: Famulski, Jakub K., Vos, Larissa, Sun, Xuejun, Chan, Gordon
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2234252/
https://www.ncbi.nlm.nih.gov/pubmed/18268100
http://dx.doi.org/10.1083/jcb.200708021
_version_ 1782150344209334272
author Famulski, Jakub K.
Vos, Larissa
Sun, Xuejun
Chan, Gordon
author_facet Famulski, Jakub K.
Vos, Larissa
Sun, Xuejun
Chan, Gordon
author_sort Famulski, Jakub K.
collection PubMed
description The mitotic checkpoint is an essential surveillance mechanism that ensures high fidelity chromosome segregation during mitosis. Mitotic checkpoint function depends on numerous kinetochore proteins, including ZW10, ROD, and Zwilch (the ROD–ZW10–Zwilch complex). Through an extensive mutagenesis screen of hZW10, we have mapped the kinetochore localization domain of hZW10 as well as the hZwint-1 interaction domain. We find that hZwint-1–noninteracting mutants still localize to kinetochores. In addition, using fluorescence recovery after photobleaching, we have found that hZW10 residency at metaphase kinetochores is brief (half-time of 13 s). However, during prometaphase or at unattached kinetochores, enhanced green fluorescent protein–hZW10 becomes a stable component of the kinetochore. Moreover, we find that stable hZW10 kinetochore residency at prometaphase kinetochores is dependent on its interaction with hZwint-1, and is essential for mitotic checkpoint arrest.
format Text
id pubmed-2234252
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-22342522008-08-11 Stable hZW10 kinetochore residency, mediated by hZwint-1 interaction, is essential for the mitotic checkpoint Famulski, Jakub K. Vos, Larissa Sun, Xuejun Chan, Gordon J Cell Biol Research Articles The mitotic checkpoint is an essential surveillance mechanism that ensures high fidelity chromosome segregation during mitosis. Mitotic checkpoint function depends on numerous kinetochore proteins, including ZW10, ROD, and Zwilch (the ROD–ZW10–Zwilch complex). Through an extensive mutagenesis screen of hZW10, we have mapped the kinetochore localization domain of hZW10 as well as the hZwint-1 interaction domain. We find that hZwint-1–noninteracting mutants still localize to kinetochores. In addition, using fluorescence recovery after photobleaching, we have found that hZW10 residency at metaphase kinetochores is brief (half-time of 13 s). However, during prometaphase or at unattached kinetochores, enhanced green fluorescent protein–hZW10 becomes a stable component of the kinetochore. Moreover, we find that stable hZW10 kinetochore residency at prometaphase kinetochores is dependent on its interaction with hZwint-1, and is essential for mitotic checkpoint arrest. The Rockefeller University Press 2008-02-11 /pmc/articles/PMC2234252/ /pubmed/18268100 http://dx.doi.org/10.1083/jcb.200708021 Text en Copyright © 2008, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Famulski, Jakub K.
Vos, Larissa
Sun, Xuejun
Chan, Gordon
Stable hZW10 kinetochore residency, mediated by hZwint-1 interaction, is essential for the mitotic checkpoint
title Stable hZW10 kinetochore residency, mediated by hZwint-1 interaction, is essential for the mitotic checkpoint
title_full Stable hZW10 kinetochore residency, mediated by hZwint-1 interaction, is essential for the mitotic checkpoint
title_fullStr Stable hZW10 kinetochore residency, mediated by hZwint-1 interaction, is essential for the mitotic checkpoint
title_full_unstemmed Stable hZW10 kinetochore residency, mediated by hZwint-1 interaction, is essential for the mitotic checkpoint
title_short Stable hZW10 kinetochore residency, mediated by hZwint-1 interaction, is essential for the mitotic checkpoint
title_sort stable hzw10 kinetochore residency, mediated by hzwint-1 interaction, is essential for the mitotic checkpoint
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2234252/
https://www.ncbi.nlm.nih.gov/pubmed/18268100
http://dx.doi.org/10.1083/jcb.200708021
work_keys_str_mv AT famulskijakubk stablehzw10kinetochoreresidencymediatedbyhzwint1interactionisessentialforthemitoticcheckpoint
AT voslarissa stablehzw10kinetochoreresidencymediatedbyhzwint1interactionisessentialforthemitoticcheckpoint
AT sunxuejun stablehzw10kinetochoreresidencymediatedbyhzwint1interactionisessentialforthemitoticcheckpoint
AT changordon stablehzw10kinetochoreresidencymediatedbyhzwint1interactionisessentialforthemitoticcheckpoint